五爪金龙叶绿体基因组特征分析  

Characteristics of the Chloroplast Genome of Ipomoea cairica

作　　者：黄琼林 陈依虹 叶晓霞 谭靖怡 梁秋婷 吴民华 HUANG Qionglin;CHEN Yihong;YE Xiaoxia;TAN Jingyi;LIANG Qiuting;WU Minhua(School of Basic Medicine,Guangdong Medical University,Zhanjiang,Guangdong,524023,China;The First Clinical Medical College,Guangdong Medical University,Zhanjiang,Guangdong,524023,China;School of Pharmacy,Guangdong Medical University,Zhanjiang,Guangdong,524023,China)

机构地区：[1]广东医科大学基础医学院,广东湛江524023 [2]广东医科大学第一临床医学院,广东湛江524023 [3]广东医科大学药学院,广东湛江524023

出　　处：《广西科学》2024年第3期451-458,共8页Guangxi Sciences

基　　金：广东省基础与应用基础研究基金项目(2018A030310116);广东省大学生创新创业训练计划项目(S202310571113);广东医科大学大学生创新创业训练计划项目(GDMU2022113,GDMU2022125)资助。

摘　　要：为了阐明药用植物五爪金龙Ipomoea cairica的叶绿体基因组特征,选用五爪金龙叶片为样品,采用试剂盒法提取DNA并建立测序文库,随后使用新一代细胞器基因组测序技术进行高通量测序,并利用生物信息学手段进行序列组装和特征分析。结果显示,五爪金龙叶绿体基因组为环状双链四分体分子,全长161780 bp,由88205 bp的大单拷贝区(Large Single Copy,LSC)、30747 bp的反向重复区(Inverted Repeat,IR)A、12081 bp的小单拷贝区(Small Single Copy,SSC)和30747 bp的反向重复区B(IRB)排列而成,含有131个基因;共有28757个密码子,偏好使用以A或T结尾的密码子;存在67个简单重复序列(Simple Sequence Repeat,SSR),并以A/T单核苷酸重复居多。五爪金龙与近缘植物在IRB-SSC边界之间存在较为明显的变异,与同属三裂叶薯I.triloba、番薯I.batatas等的亲缘关系更近。本研究报道了五爪金龙叶绿体基因组的结构和序列组成,并分析其特征,为五爪金龙的物种鉴定、遗传进化及开发利用提供了基因组信息。To clarify the chloroplast genome features of Ipomoea cairica,this study used a kit to extract DNA from the leaves of I.cairica and then constructed the sequence library.The next generation sequencing was employed for high throughput organelle sequencing,and then bioinformatics tools were used to assemble and analyze the sequences.The results showed that the chloroplast genome of I.cairica was a circular quadripartite structure with a total length of 161780 bp,which was composed of a Large Single Copy(LSC,88205 bp),an Inverted Repeat A(IRA,30747 bp),a Small Single Copy(SSC,12081 bp),and an Inverted Repeat B(IRB,30747 bp).A total of 131 genes were embodied in the chloroplast genome.The genome contained 28757 codons,among which those ended with A/T were frequently used.There were 67 Simple Sequence Repeats(SSR),which were dominated by the mononucleotide repeat A/T.I.cairica showed significant variations with related species at the boundary between IRB and SSC and shared close relationship with I.triloba and I.batatas.This work reports and characterizes the chloroplast genome of I.cairica,which will provide genome data for species identification,phylogenetic relationship research,and development and utilization of this herbal medicine.

关 键 词：五爪金龙 叶绿体基因组 密码子偏好性 简单重复序列 系统进化关系 

分 类 号：R282[医药卫生—中药学]