基于同步荧光技术的牛肉中掺杂猪肉鉴别方法研究  

Potentiality of Synchronous Fluorescence Technology for Identification of Pork Adulteration in Beef

作　　者：李月 林义利[4] 周云云 杨信廷 王增利[1] 刘欢 LI Yue;LIN Yi-li;ZHOU Yun-yun;YANG Xin-ting;WANG Zeng-li;LIU Huan(Department of Food Nutrition and Safety,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Information Technology Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China;National Engineering Research Center for Agri-Product Quality and Safety Traceability,Beijing 100097,China;Pinggu Garden Management Committee of Zhongguancun Science and Technology Park,Beijing 101200,China)

机构地区：[1]中国农业大学食品科学与营养工程学院食品营养与安全系,北京100083 [2]北京市农林科学院信息技术研究中心,北京100097 [3]农产品质量安全追溯技术及应用国家工程研究中心,北京100097 [4]中关村科技园区平谷园管理委员会,北京101200

出　　处：《光谱学与光谱分析》2024年第10期2968-2972,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(32001775)资助。

摘　　要：牛肉是我国重要的食用肉品类,近年来随着人们对牛肉需求的不断增加,将猪肉冒充或添加在牛肉中出售的现象也日益严重,亟需简单、快速的检测肉品掺假的方法。首先分析了牛肉和猪肉的三维荧光光谱特征差异,确定同步荧光的波长差;采用激发-发射固定波长差为160 nm的同步荧光光谱,对牛肉掺杂猪肉的情况进行了定性判别和定量分析。以测试集、验证集和预测集样本的判别正确率作为定性判别模型的评价指标;以相关系数(r)、校正均方根误差(RMSEC)和预测均方根误差(RMSEP)作为定量分析模型的评价指标。实验结果表明:牛肉和猪肉的三维荧光光谱有显著差异,牛肉在Ex/Em为270/320、330/400、350/500、430/515和410/570 nm处有荧光峰,猪肉的三个荧光峰分别在Ex/Em为270/320、330/400和430/515 nm处。设置同步荧光波长差为160 nm,能采集到牛肉的3个荧光峰,且其中两个位于峰顶。牛肉、猪肉、掺假肉SVM定性判别模型的校正集准确率为97.56%,预测准确率可达92.31%。对比了无处理、MSC处理和SNV处理的牛肉中猪肉掺加量PLS预测模型,无处理的PLS模型最优,其rc、rp、RMSEC和RMSEP分别达到0.9786、0.9590、0.0597和0.0927。基于同步荧光技术结合SVM和PLS的牛肉掺假猪肉定性判别和定量分析检测模型具有较高识别率和检测精度,可以较为准确、快速地检测牛肉中是否掺杂猪肉。Beef is an important edible meat in China.In recent years,with the increasing demand for beef,the phenomenon of pork being impersonated or added to beef for sale has become increasingly serious,and there is an urgent need for simple and rapid detection methods to monitor adulteration behavior.This study analyzed the three-dimensional fluorescence spectra of beef and pork to determine the wavelength difference of synchronous fluorescence.A synchronous fluorescence spectrum with a fixed wavelength difference of 160 nm was used to qualitatively distinguish and quantitatively analyze the doping of beef with pork.The discriminant accuracy of the test set,verification set,and prediction set samples are taken as the evaluation index of the qualitative Discriminative model:Correlation coefficient(r),corrected Root-mean-square deviation(RMSEC)and predicted Root-mean-square deviation(RMSEP)were used as the evaluation indicators of the quantitative analysis model.The experimental results show a significant difference in the three-dimensional fluorescence spectra between beef and pork.Beef has fluorescence peaks at Ex/Em values of 270/320,330/400,350/500,430/515 and 410/570 nm,while pork has three fluorescence peaks at Ex/Em values of 270/320,330/400 and 430/515 nm.By setting the synchronous fluorescence wavelength difference to 160 nm,three fluorescence peaks of beef can be collected,with two of them located at the peak.The correction set accuracy of the SVM qualitative Discriminative model for beef,pork,and adulterated meat was 97.56%,and the prediction accuracy was 92.31%.The PLS prediction model for pork addition in beef without treatment,MSC treatment,and SNV treatment was compared.The PLS model without treatment was the best,with rc,rp,RMSEC,and REMSP reaching 0.9786,0.9590,0.0597,and 0.0927,respectively.Therefore,the qualitative discrimination and quantitative analysis detection model for beef adulterated pork based on synchronous fluorescence technology combined with SVM and PLS has a high recognition rate and detect

关 键 词：牛肉 掺假 猪肉 同步荧光 

分 类 号：TS207.3[轻工技术与工程—食品科学]