适合于个性化培养的红茶菌发酵综合性实验  

Comprehensive experiment on the fermentation of kombucha suitable for personalized cultivation

作　　者：刘斌杰 杨民和[2] LIU Binjie;YANG Minhe(Department of Medicine and Nursing,Sanming Vocational Technical College,Sanming 365000,China;School of Life Sciences,Fujian Normal University,Fuzhou 350117,China)

机构地区：[1]三明医学科技职业学院医学与护理系,福建三明365000 [2]福建师范大学生命科学学院,福建福州350117

出　　处：《高师理科学刊》2024年第9期106-110,共5页Journal of Science of Teachers＇College and University

基　　金：三明市引导性项目(2022-S-39);三明市职业院校联盟中青年教师教育科研项目(smyx2023008)。

摘　　要：为推动高等教育教学改革,以传统食品红茶菌发酵为素材设计综合性实验内容,培养本科生对日常生活的洞察能力和探究性学习精神,达到个性化培养目标.该综合性实验适合生物科学、生物技术、生物工程和食品工程等专业学生作为人才培养的实验内容.实验内容多学科交叉,涉及微生物分离、微生物培养、酸碱滴定、高效液相色谱、顶空固相微萃取和GC-MS等实验技术.采用平时表现、实验报告和毕业论文等方式考核学生的成绩.实验内容激发了学生的学习积极性,提高了学生的实验技能和独立工作能力.通过三年的建设,该设计在实验内容、实施方式和考核措施等方面均已形成了一定的特色,符合素质教育和能力培养的学科发展主流方向.To promote reform in higher education pedagogy,a comprehensive experimental curriculum has been designed using the fermentation of traditional kombucha as its subject matter.This approach aims to cultivate undergraduate students'observational skills in daily life and foster a spirit of inquiry-based learning,thereby achieving personalized educational objectives.This integrative experiment is suitable as a component of talent cultivation for students majoring in biological sciences,biotechnology,bioengineering and food engineering.The experimental contents were interdisciplinary,involving microbe isolation,culture,titrimetric analysis,headspace solid phase micro-extraction,HPLC and GC-MS analysis,etc.Student performance is evaluated through multiple assessment methods,including ongoing performance,laboratory reports and graduation theses.This experimental design has effectively stimulated students'enthusiasm for learning and enhanced their laboratory skills and capacity for independent work.Over a three-year development period,this curriculum has established distinctive features in terms of experimental content,implementation methodology and assessment measures.It aligns with the mainstream disciplinary trends of quality education and competency development.

关 键 词：个性化培养 综合性实验 红茶菌 本科生 

分 类 号：Q93[生物学—微生物学] G642.0[文化科学—高等教育学]