响应面法优化超声辅助提取大果沙枣叶多酚工艺及其抗氧化和抗糖化能力研究  

Optimization of Ultrasonic-Assisted Extraction Process of Polyphenols from Leaves of Elaeagnus moorcroftii Wall.by Response Surface Methodology and Study on Their Antioxidant and Anti-Glycation Capacity

作　　者：孙妍 高尚 郭玉婷 陈璐 薛雪 唐辉[2] 廖兵武 贾瑞博 SUN Yan;GAO Shang;GUO Yu-ting;CHEN Lu;XUE Xue;TANG Hui;LIAO Bing-wu;JIA Rui-bo(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;School of Pharmacy,Shihezi University,Shihezi 832003,China;College of Food Science and Engineering,Tarim University,Alar 843300,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]石河子大学药学院,新疆石河子832003 [3]塔里木大学食品科学与工程学院,新疆阿拉尔843300

出　　处：《中国调味品》2024年第10期186-193,共8页China Condiment

基　　金：广东省自然科学基金面上项目(2024A1515010576);新疆植物药资源利用教育部重点实验室主任基金(2021QDJF002)。

摘　　要：在推动循环经济的进程中,该研究着重于大果沙枣叶多酚的提取及其应用潜力。利用超声波辅助提取技术对大果沙枣叶中的多酚类化合物进行了提取工艺的优化,并对其抗氧化和抗糖化能力进行了全面评估。试验确定了最佳提取条件为超声时间20.00 min、料液比1∶53.40(g/mL)、超声温度69.70℃、超声功率400 W,此条件下得到多酚提取量为(28.24±0.46)mg/g。抗氧化试验表明,该多酚对DPPH·、·OH和ABTS^(+)·具有较强清除能力,其IC_(50)值分别为0.112,0.110,0.146 mg/mL。在BSA-GLC模拟反应体系下,其糖化抑制率显著提高至(44.64±7.20)%。研究结果表明,超声辅助提取的大果沙枣叶多酚具有显著的体外抗氧化和抗糖化能力,其能力随着大果沙枣叶多酚浓度的增加而增强,表明其可作为潜在的天然食品添加剂,为沙枣副产品的综合利用提供了新方向。In the process of promoting circular economy,the extraction of polyphenols from Elaeagnus moorcroftii Wall.leaves and their application potential are focused on in this study.The extraction process of polyphenols from the leaves of Elaeagnus moorcroftii Wall.is optimized by ultrasonic-assisted extraction technology,and their antioxidant and anti-glycation abilities are evaluated comprehensively.The optimal extraction conditions are determined as ultrasonic time of 20.00 min,solid-liquid ratio of 1∶53.40(g/mL),ultrasonic temperature of 69.70℃,ultrasonic power of 400 W.Under such conditions,the extraction amount of polyphenols is(28.24±0.46)mg/g.Antioxidant tests show that the polyphenols have stronger scavenging abilities on DPPH·,·OH and ABTS^(+)·,and their IC_(50) values are 0.112,0.110,0.146 mg/mL respectively.Under the BSA-GLC simulated reaction system,the inhibition rate of glycosylation significantly increases to (44.64±7.20)%. The research results show that the polyphenols extracted by ultrasonic-assisted extraction technology have significant antioxidant and anti-glycation abilities in vitro , and their abilities increase with the increase of concentration of polyphenols from Elaeagnus moorcroftii Wall. leaves, which indicates that they could be used as potential natural food additives, and has provided a new direction for the comprehensive utilization of by-products of Elaeagnus moorcroftii Wall..

关 键 词：大果沙枣叶 多酚 超声辅助提取 响应面 抗氧化 美拉德反应 

分 类 号：TS201.1[轻工技术与工程—食品科学]