紫色野生樱桃李提取物抗氧化和降血糖活性研究及化学成分鉴定  

Antioxidant and hypoglycemic activities of purple Prunus cerasifera Ehrh extracts and identification of chemical constituents

作　　者：罗世博 刘伟 谢星 冯亚萍 彭春彦 张露[3] LUO Shibo;LIU Wei;XIE Xing;FENG Yaping;PENG Chunyan;ZHANG Lu(Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources,School of Chemistry and Chemical Engineering,Yili Normal University,Yining 835000,China;College of Health,Jiangxi Normal University,Nanchang 330022,China;National R&D center of Freshwater Fish Processing,College of Life Science,Jiangxi Normal University,Nanchang 330022,China)

机构地区：[1]伊犁师范大学化学化工学院,新疆生物质资源清洁转化与高值利用重点实验室,新疆伊宁835000 [2]江西师范大学健康学院,江西南昌330022 [3]江西师范大学生命科学学院,国家淡水鱼加工高技术研发专业中心,江西南昌330022

出　　处：《食品与发酵工业》2024年第19期191-200,I0003,I0004,共12页Food and Fermentation Industries

基　　金：新疆维吾尔自治区自然科学基金项目(2022D01C455);伊犁师范大学提升学科综合实力专项自科重点项目(22XKZZ08)。

摘　　要：野生樱桃李富含多酚类化合物,具有“雪域珍果”之称,但其抗糖尿病活性和主要成分不明。该文以紫色野生樱桃李(purple Prunus cerasifera Ehrh.,PPE)为研究对象,采用体积分数70%乙醇提取和溶剂萃取法提取和富集多酚类化合物,通过自由基清除能力、酶抑制能力、促进人肝癌细胞(HepG2细胞)葡萄糖消耗及抗糖基化能力实验评价醇提物和萃取组分的抗氧化和降血糖活性,通过液质联用技术鉴定主要化学组成。结果表明,PPE的乙酸乙酯相(ethyl acetate fraction,EAF)具有最高的总酚(219.27 mg GAE/g E)和总黄酮含量(165.58 mg QuE/g E),以及最强的ABTS阳离子自由基清除能力、α-葡萄糖苷酶抑制能力、抗糖基化能力和HepG2胰岛素抵抗细胞模型细胞葡萄糖消耗能力,其中α-葡萄糖苷酶抑制能力为对照品阿卡波糖的31倍。PPE的正丁醇相具有最高的DPPH自由基清除能力和二肽基肽酶-4抑制活性。采用高效液相色谱飞行时间质谱联用(HPLC-quadrupole time-of-flight tandem mass spectrometry,HPLC-QTOF-MS/MS)技术从EAF组分中鉴定出43个化合物,包括12个有机酸、9个酚酸、16个黄酮类化合物和6个其他化合物。因此,乙酸乙酯和正丁醇是萃取富集野生樱桃李中活性成分的适宜溶剂,酚酸和黄酮类化合物是PPE中的主要抗氧化和降血糖成分,研究结果可为PPE在降血糖营养食品或膳食补充剂方面的应用提供理论指导。Prunus cerasifera Ehrh is rich in polyphenols and named‘precious fruit in the snow region’,but the anti-diabetic activity and its bioactive compounds remain unclear.In this study,70%ethanol extraction and solvent fraction were applied to extract the polyphenols in purple PE(PPE).The antioxidant and hypoglycemic activities were evaluated by radical scavenging ability,enzymes inhibition ability,promotion of glucose consumption in HepG2 cells and anti-glycation ability assays.The major bioactive compounds were identified by liquid chromatography-mass spectroscopy technique.Results indicated that the ethyl acetate fraction of PPE showed the highest total phenolics(219.27 mg GAE/g E)and total flavonoids(165.58 mg QuE/g E)content,along with the strongest ABTS cation radical scavenging ability(163.36μg/mL),α-glucosidase inhibition activity(28.95μg/mL),promotion glucose absorption in HepG2 cells and anti-glycation abilities.Theα-glucosidase inhibition was 31 times higher than that of acarbose.The n-butanol fraction of PPE showed the highest DPPH radical scavenging and DPP-4 enzyme inhibitory capacity.Forty-three compounds were identified from EAF by HPLC-QTOF-MS/MS,including 12 organic acids,9 phenolic acids,16 flavonoids and 6 other class of compounds.Therefore,ethyl acetate and n-butanol are the suitable solvents to extract active ingredients from PPE,phenolic acids and flavonoids are the major antioxidants and hypoglycemic compounds.This study could provide theoretical guidance for the application of PPE in hypoglycemic nutritional foods or dietary supplements.

关 键 词：野生樱桃李 多酚 抗氧化活性 降血糖活性 鉴定 

分 类 号：TS201.4[轻工技术与工程—食品科学]