植物乳杆菌对猪肉中单核细胞增生李斯特氏菌抑制模型的建立  

Modeling the Inhibitory Effect of Lactobacillus plantarum on the Growth of Listeria monocytogenes in Pork

作　　者：张文敏 董庆利[2] 刘阳泰[2] 辛宝 钱文文 ZHANG Wenmin;DONG Qingli;LIU Yangtai;XIN Bao;QIAN Wenwen(School of Public Health,Shaanxi University of Chinese Medicine,Xianyang 712046,China;School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]陕西中医药大学公共卫生学院,陕西咸阳712046 [2]上海理工大学健康科学与工程学院,上海200093

出　　处：《肉类研究》2024年第10期1-8,共8页Meat Research

基　　金：陕西省科技厅一般项目(2024JC-YBQN-0236);陕西中医药大学博士科研启动基金项目(303-124020069);大学生创新创业训练计划资助项目(S202410716005)。

摘　　要：人类患李斯特菌病的概率和食品中单核细胞增生李斯特氏菌的最大污染浓度(Nmax)紧密相关。益生菌作为生物保护菌应用在食品中控制单核细胞增生李斯特氏菌的生长已得到广泛应用,在复杂的食品环境中,益生菌对单核细胞增生李斯特氏菌生长特性的影响可通过细菌间的竞争抑制模型来描述。本研究首先确认益生菌植物乳杆菌植物亚种CICC6257(以下简称为植物乳杆菌CICC6257)在猪肉中的生长能力,探究该菌对猪肉品质的影响,然后对选择性培养基的菌落计数能力进行验证,最后基于预测微生物学方法研究植物乳杆菌CICC6257对猪肉中单核细胞增生李斯特氏菌生长特性的影响。结果表明:植物乳杆菌CICC6257在13℃贮藏的猪肉中生长良好,60h左右生长达到稳定期,并且在货架期内对猪肉的pH值无显著影响(P≥0.05);选择性培养基可以很好地对单核细胞增生李斯特氏菌和植物乳杆菌CICC6257进行分离计数;Jameson-effect模型能够准确描述植物乳杆菌CICC6257对单核细胞增生李斯特氏菌的抑制作用;植物乳杆菌CICC6257对单核细胞增生李斯特氏菌的迟滞时间(λ)和最大比生长速率(μmax)无显著影响(P≥0.05),但可将猪肉中单核细胞增生李斯特氏菌的Nmax显著降低0.75(lg(CFU/g))(P<0.05)。The maximum population density(Nmax)of Listeria monocytogenes in foods plays an important role in the probability of suffering listeriosis.Probiotics are widely used as bioprotective culture in foods to control the growth of L.monocytogenes.In complex food environments,the impact of probiotic on the growth characteristics of L.monocytogenes can be described by a model describing inter-bacteria competitive inhibition.In this study,we first confirmed the growth ability of Lactobacillus plantarum CICC6257 in pork and explored its effect on pork quality.Then,we validated the accuracy of microbial counting results with selective culture medium.Finally,the antagonistic ability of L.plantarum CICC6257 against L.monocytogenes was investigated on the basis of predictive microbiology.Results showed that L.plantarum CICC6257 could grow well in pork and reach the stationary phase after 60 h at 13℃.L.plantarum CICC6257 had no significant effect on the pH of pork within the shelf-life period(P≥0.05).Moreover,both species were well isolated by the selective medium.The Jameson-effect model accurately described the inhibitory effect of L.plantarum CICC6257 on L.monocytogenes in pork.The presence of L.plantarum CICC6257 showed no significant effect on the lag phase(λ)or maximal growth rate(μmax)of L.monocytogenes(P≥0.05),but decreased the Nmax significantly by 0.75(lg(CFU/g))(P<0.05).

关 键 词：植物乳杆菌CICC6257 猪肉 单核细胞增生李斯特氏菌 抑制模型 

分 类 号：TS201.3[轻工技术与工程—食品科学]