高效液相色谱-串联质谱法测定食品中7种红曲色素  

Determination of 7 kinds of Monascus pigments in food by high performance liquid chromatography-tandem mass spectrometry

作　　者：张蕤 郭舒岗[1] 张春萍 熊慧霞 薛红波 ZHANG Rui;GUO Shu-Gang;ZHANG Chun-Ping;XIONG Hui-Xia;XUE Hong-Bo(Shanxi Provincial Center for Disease Control and Prevention,Taiyuan 030000,China)

机构地区：[1]山西省疾病预防控制中心,太原030000

出　　处：《食品安全质量检测学报》2024年第18期281-289,共9页Journal of Food Safety and Quality

基　　金：山西省卫生健康委科研项目(2023132)。

摘　　要：目的建立通过式固相萃取-高效液相色谱-串联质谱法测定食品中7种红曲色素。方法样品经过乙腈提取,Oasis PRiME HLB固相萃取柱(200 mg,6 cc)净化,Waters ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱分离,采用高效液相色谱串联质谱仪检测,基质匹配外标法定量。并对豆制品、糕点、酸奶、腐乳、熟肉、饮料以及腊肠7种基质中红曲色素回收率进行了考察。结果7种基质中潘红胺、红曲红胺、红曲素、红斑素、红曲黄素、红曲玉红素、红曲红素的加标回收率分别为73.9%~112.1%,70.1%~92.6%,72.4%~103.4%,73.8%~93.5%,73.4%~113.3%,71.6%~105.7%,75.0%~112.1%;7种色素的相对标准偏差为0.2%~15.7%;红曲黄素的检出限为3.0μg/kg,其余6种色素的检出限为1.0μg/kg。结论该方法前处理简单快速、灵敏度高、准确度和精密度良好,采用基质匹配的标准曲线定量,适用于不同基质中7种红曲色素的测定。Objective To establish a method for the determination of 7 kinds of Monascus pigments in food by solid phase extraction-high performance liquid chromatography-tandem mass spectrometry.Methods The samples were extracted by acetonitrile,purified by Oasis PRiME HLB solid phase extraction column(200 mg,6 cc),separated by Waters ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)column,and detected by high performance liquid chromatography tandem mass spectrometer,and quantified by matrix matching external standard method.The recovery rates of red yeast pigment in 7 kinds of matrices such as soy products,pastries,yogurt,fermented bean curd,cooked meat,beverages,and sausages was examined.Results In the validation,the recoveries of rubropunctamine,Monascus amine,monascin,rubropunctation,ankaflavin,monascorubrin and Monascus red in 7 kinds of matrices were 73.9%-112.1%,70.1%-92.6%,72.4%-103.4%,73.8%-93.5%,73.4%-113.3%,71.6%-105.7%,75.0%-112.1%,respectively,the relative standard deviations of 7 kinds of pigments were 0.2%-15.7%.The limits of detection of ankaflavin was 3.0μg/kg,and the limit of detection of the other 6 kinds of pigments was 1.0μg/kg.Conclusion The method is simple,rapid,sensitive,accurate and precise,and can be used to detect 7 kinds of monascus pigments in different substrates.

关 键 词：红曲色素 高效液相色谱-串联质谱法 食品添加剂 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]