栀子花乙酸乙酯萃取物对副溶血性弧菌抑菌活性及机理研究  

Bacteriostatic activity and mechanism of ethyl acetate extract from Gardenia jasminoides Ellis flower against Vibrio parahaemolyticus

作　　者：胡钰 张齐杰 傅纤雯 王艳[1] 许光治[1] 张有做[1,2] 倪勤学 HU Yu;ZHANG Qijie;FU Qianwen;WANG Yan;XU Guangzhi;ZHANG Youzuo;NI Qinxue(Food and Health College,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Jiaozhi Technology Co.Ltd.,Hangzhou 311300,China)

机构地区：[1]浙江农林大学食品与健康学院,浙江杭州311300 [2]浙江骄栀科技有限公司,浙江杭州311300

出　　处：《食品与发酵工业》2024年第21期51-59,共9页Food and Fermentation Industries

基　　金：浙江省大学生科技活动计划暨新苗人才计划资助项目(2022R412A036)。

摘　　要：为探究栀子花乙酸乙酯相(Gardenia jasminoides Eills flower-ethyl acetate extract,GFE-EA)对副溶血性弧菌(Vibrio parahemolyticus)的抑菌活性及机理,测定了GFE-EA对V.parahemolyticus的最低抑菌浓度(minimal inhibit concentration,MIC)和最低杀菌浓度(minimum bactericidal concentration,MBC)。通过对其生长曲线、细胞内容物泄露、细胞壁膜通透性、流动性和完整性、胞内酶活性,三磷酸腺苷(adenosine triphosphate,ATP)、丙二醛(malondialdehyde,MDA)和活性氧(reactive oxygen species,ROS)含量等的影响来探究其可能存在的抑菌机理。结果表明,GFE-EA对V.parahemolyticus的MIC和MBC分别达到2.5、20 mg/mL。与高于MIC浓度的GFE-EA共培养,发现细菌的生长受到明显抑制,上清液中核酸和蛋白质含量有显著的增加,扫描电镜发现菌体细胞形态变化明显,细胞膜流动性、通透性和完整性变差,V.parahemolyticus胞内抗氧化酶及ATP酶活性均呈下降趋势,MDA含量上升,ROS水平升高,并且其DNA荧光强度变弱,电泳条带弥散。说明GFE-EA能够通过延迟缩短细菌生长的对数期,破坏细菌细胞壁膜系统和细胞形态,影响胞内能量代谢,破坏菌体遗传物质等来抑制细菌的生长繁殖,甚至导致其死亡。该研究为探明GFE-EA抑制食源性病原菌的机理奠定了理论基础,表明栀子花在天然食品防腐剂领域中具有广泛的开发应用潜力。This study aimed to investigate the bacteriostatic activity and mechanism of GFE-EA from Gardenia jasminoides Eills flower against Vibrio parahaemolyticus.The study determined the minimum inhibitory concentration and minimum bactericidal concentration of GFE-EA against V.parahemolyticus.The possible antibacterial mechanism was explored by growth curve,cell content leakage,cell wall and membrane permeability,fluidity and integrity,intracellular enzyme activity,adenosine triphosphate,malondialdehyde,reactive oxygen species(ROS)content,and intracellular DNA structure.Results showed that the MIC and MBC reached 2.5 mg/mL and 20 mg/mL of GFE-EA against V.parahemolyticus,respectively.When co-cultured with GFE-EA of higher than MIC,it was found that the growth of bacteria was significantly inhibited and the contents of nucleic acid and protein in the supernate were significantly increased.The morphology of bacterial cells was significantly changed by scanning electron microscopy.The fluidity,permeability,and integrity of cell membranes deteriorated.The activity of antioxidant enzymes and ATPase in V.parahemolyticus decreased,MDA content increased and ROS level increased.The DNA fluorescence intensity was weakened and the bands dispersed,which could achieve the purpose of inhibiting bacterial growth and death by delaying and shortening the logarithmic growth period of bacteria,damaging bacterial cell wall membrane system and cell morphology,affecting their energy metabolism,and destroying bacterial genetic material.This study laid a theoretical foundation for exploring the mechanism of GFE-EA inhibiting food-borne pathogens,indicating that Gardenia jasminoides Ellis has a wide potential for development and application in the field of natural food preservatives.

关 键 词：栀子花 副溶血性弧菌 抑菌机理 扫描电子显微镜 DNA损伤 

分 类 号：TS201.3[轻工技术与工程—食品科学]