不同乙醇浓度手掌参提取物挥发性成分及抗氧化作用分析  

Analysis of Volatile Components and Antioxidant Effects of the Different Ethanol Concentrations of Gymnadenia conopsea(L.)R.Br.

作　　者：陈天荣 刘家乐 CHEN Tianrong;LIU Jiale(Tibet Academy of Agricultural and Animal Husbandry Sciences,Food Science Institute,Lhasa 850000,China)

机构地区：[1]西藏自治区农牧科学院农产品开发与食品科学研究所,西藏拉萨850000

出　　处：《食品安全导刊》2024年第30期55-63,共9页China Food Safety Magazine

基　　金：西藏自治区自然科学基金(XZ202201ZR0015G);西藏居民膳食营养健康改善与优化农产品保供科技支撑(54000024210200019411);西藏自治区科技重大专项(XZ202201ZD0001N);西藏财政项目(XZNKYSPS-2024-C-045)。

摘　　要：为探究不同乙醇浓度对手掌参挥发性成分种类及抗氧化能力的影响,本实验采用顶空-气相色谱-质谱法(Headspace-Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry,HS-SPME-GC-MS)对浓度为75%和95%手掌参乙醇提取物及手掌参原料的挥发性成分进行测定,采用酶标法对两种手掌参乙醇提取物的总抗氧化能力(Total Antioxidant Capacity,T-AOC)、DPPH自由基和超氧阴离子自由基(O_(2)^(-)·)清除能力进行评估。结果显示,原料及两种提取物中共检测到82种挥发性物质。其中,95%手掌参乙醇提取物的挥发性成分种类最多,为56种;两种手掌参乙醇提取物共有的挥发性成分为16种,差异成分4种;95%手掌参乙醇提取物的T-AOC及DPPH自由基清除能力显著高于75%手掌参乙醇提取物,但O_(2)^(-)·清除率显著低于75%手掌参乙醇提取物;对甲酚、十六烷酸乙酯、苯乙烯和辛醇等提取物挥发性成分与T-AOC及DPPH自由基清除能力呈显著正相关,与O_(2)^(-)·清除能力呈显著负相关。本研究可为手掌参挥发性功能成分的开发提供参考。In order to investigate the effects of different ethanol concentrations on the types of volatile components and antioxidant capacity of Gymnadenia conopsea(L.)R.Br.,this experiment used headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)to determine the volatile components of Gymnadenia conopsea(L.)R.Br.ethanol extracts and Gymnadenia conopsea(L.)R.Br.raw materials at concentrations of 75%and 95%.Enzyme linked immunosorbent assay(ELISA)was used to evaluate the total antioxidant capacity(T-AOC),DPPH radical and superoxide anion radical(O_(2)^(-)·)scavenging ability of the two Gymnadenia conopsea(L.)R.Br.ethanol extracts.The results showed that a total of 82 volatile substances were detected in the raw materials and two extracts.Among them,95%of the volatile components in the ethanol extract of Gymnadenia conopsea(L.)R.Br.are the most diverse,with 56 types;there are 16 common volatile components and 4 different components in the ethanol extracts of two types of Gymnadenia conopsea(L.)R.Br.;the T-AOC and DPPH free radical scavenging ability of 95%Gymnadenia conopsea(L.)R.Br.ethanol extract was significantly higher than that of 75%Gymnadenia conopsea(L.)R.Br.ethanol extract,but the O_(2)^(-)·scavenging rate was significantly lower than that of 75%Gymnadenia conopsea(L.)R.Br.ethanol extract;the volatile components of extracts such as cresol,ethyl hexadecanoate,styrene,and octanol were significantly positively correlated with T-AOC and DPPH radical scavenging ability,and significantly negatively correlated with O_(2)^(-)·scavenging ability.This study can provide reference for the development of volatile functional components in the Gymnadenia conopsea(L.)R.Br..

关 键 词：手掌参 乙醇提取物 挥发性成分 抗氧化能力 

分 类 号：TS201.2[轻工技术与工程—食品科学]