不同品种桑椹发酵酒的体外抗氧化、降血糖及降血脂活性研究  

Study on in vitro antioxidant,hypoglycemic,and hypolipidemic activities of mulberry fermented wine brewed with different varieties

作　　者：黄越 黄传书 杨碧文 赵珮 王梅 周鸿媛[2] 刘艳 吴均 戴宏杰 HUANG Yue;HUANG Chuanshu;YANG Biwen;ZHAO Pei;WANG Mei;ZHOU Hongyuan;LIU Yan;WU Jun;DAI Hongjie(Chongqing Sericulture Science and Technology Research Institute,Chongqing 400700,China;College of Food Science,Southwest University,Chongqing 400715,China)

机构地区：[1]重庆市蚕业科学技术研究院,重庆400700 [2]西南大学食品科学学院,重庆400715

出　　处：《食品与发酵工业》2024年第22期150-158,共9页Food and Fermentation Industries

基　　金：重庆市科研机构绩效激励引导专项(cstc2022jxjl00015);重庆市技术创新与应用发展专项重点项目(CSTB2022TIAD-KPX0081);国家自然科学基金项目(31901683)。

摘　　要：以大10、中桑5801、嘉陵30和紫金6号4个品种桑椹为原料酿制发酵酒,研究其体外抗氧化、降血糖及降血脂活性,并对不同品种桑椹及其发酵酒的理化指标(如总酚、花色苷含量等)进行了对比分析。结果表明,大10桑椹发酵酒的总酚(2.44 mg GAE/mL)和花色苷含量(410.79 mg CGE/L)显著高于其他3个品种桑椹发酵酒。抗氧化活性方面,大10桑椹发酵酒的DPPH自由基清除率(99.70%)与铁离子还原能力均为最高。嘉陵30、中桑5801与大10桑椹发酵酒的ABTS阳离子自由基清除率均超过95%,显著高于紫金6号(81.29%)。大10和紫金6号桑椹发酵酒对羟自由基(·OH)的清除能力相对较高(>85%);降血糖活性方面,大10桑椹发酵酒表现出更好的效果,其对α-葡萄糖苷酶和α-淀粉酶活性抑制率分别为96.45%和68.13%;降血脂活性方面,大10桑椹发酵酒也具有相对较好的结合甘氨胆酸钠和牛磺胆酸钠能力。综上,4种桑椹发酵酒均具有较好的体外抗氧化活性、抑制α-葡萄糖苷酶活性、抑制α-淀粉酶活性和体外降血脂活性,大10桑椹发酵酒在整体上表现出更好的功能活性。相关性分析结果表明,桑椹发酵酒中的总酚含量与其降血糖活性呈极显著正相关(P<0.001),花色苷含量与其DPPH自由基清除能力、降血脂活性呈极显著正相关,总酸含量与其·OH清除能力呈极显著正相关。Fermented wine was brewed with four mulberry varieties respectively,including Da 10,Zhongsang 5801,Jialing 30,and Zijin 6.The antioxidant,hypoglycemic,and hypolipidemic activities in vitro of mulberry fermented wine were studied.Furthermore,the physicochemical indexes(e.g.,total phenol and anthocyanin contents,etc.)of different mulberry varieties and their fermented wine were comparatively analyzed.Results showed that the contents of total phenol(2.44 mg GAE/mL)and anthocyanin(410.79 mg CGE/L)in Da 10 mulberry fermented wine were significantly higher than those in the other three varieties.In terms of antioxidant activity,the DPPH radical scavenging rate(99.70%)and ferric reduction capacity of Da 10 mulberry fermented wine were the highest,and the ABTS cationic radical scavenging rates of Jialing 30,Zhongsang 5801,and Da 10 mulberry fermented wine were over 95%,which was significantly higher than that of Zijin 6(81.29%).The·OH scavenging rates of Da 10 and Zijin 6 mulberry fermented wine were relatively high(>85%).In terms of hypoglycemic activity,Da 10 mulberry fermented wine showed a better effect,and its inhibition rate ofα-glucosidase andα-amylase activity was 96.45%and 68.13%,respectively.In terms of hypolipidemic activity,Da 10 mulberry fermented wine also had a better binding ability to sodium glycocholate and sodium taurocholate.In summary,all four mulberry fermented wines had good antioxidant activity,inhibition activity ofα-glucosidase andα-amylase as well as hypolipidemic activity in vitro.On the whole,Da 10 mulberry fermented wine showed better functional activity.The results of correlation analysis showed that the content of total phenol in mulberry fermented wine was significantly positively correlated with its hypoglycemic activity(P<0.001),anthocyanin content was significantly positively correlated with DPPH radical scavenging ability and hypolipidemic activity,and total acid content was significantly positively correlated with·OH scavenging ability.

关 键 词：桑椹 发酵酒 抗氧化 降血糖 降血脂 

分 类 号：TS262.7[轻工技术与工程—发酵工程]