西藏开菲尔粒中降核苷乳酸菌的筛选及其潜在降尿酸功能  

Isolation of nucleoside-degrading lactic acid bacteria from Tibetan kefir grains and their potential uric acid lowering function

作　　者：董升辉 张滋雨 赵珈丞 张百峰 张杰[1] DONG Shenghui;ZHANG Ziyu;ZHAO Jiacheng;ZHANG Baifeng;ZHANG Jie(Qilu University of Technology(Shandong Academy of Sciences),Jinan 250000,China;Yinfu Biotechnology Co.Ltd.,Jinan 250000,China;Shandong Shuifa Life Science Research,Jinan 250000,China)

机构地区：[1]齐鲁工业大学(山东省科学院),山东济南250000 [2]因福(济南)生物科技有限公司,山东济南250000 [3]山东水发生命科学研究有限公司,山东济南250000

出　　处：《食品与发酵工业》2024年第22期226-232,共7页Food and Fermentation Industries

基　　金：齐鲁工业大学(山东省科学院)创新项目(2022JBZ01-06);2023年度省重点研发计划(乡村振兴科技创新提振行动计划)(2023TZXD003);中央引导地方科技发展专项资金项目(YDZX2023006)。

摘　　要：为了获得能够辅助治疗高尿酸血症的益生菌菌株,该研究自西藏开菲尔粒中分离获得28株乳酸菌并基于HPLC法对菌株的肌苷、鸟苷和腺苷降解能力进行研究,其中发酵粘液乳杆菌ZD103具有最高的肌苷、鸟苷和腺苷降解能力。为了进一步分析菌株ZD103的潜在降尿酸作用,基于HPLC法测定了菌株ZD103代谢物对黄嘌呤氧化酶(xanthine oxidase, XOD)的抑制作用,结果显示,菌株ZD103代谢物能够显著抑制XOD的活性且发挥抑制作用的是存在于胞外代谢物中的酸类物质。基于分子对接将代谢物中酸类物质与XOD进行对接打分,并选择分数最高的3种物质对其XOD的抑制作用进行验证,结果显示,1-羟基-2-萘甲酸、3-吲哚丙烯酸、吲哚丙酮酸这3种物质对XOD均具有不同程度的抑制作用,其中1-羟基-2-萘甲酸抑制效果最佳,对XOD的抑制率可达到(98.66±1.63)%。该研究为预防和治疗高尿酸血症益生菌产品的开发提供了理论依据和优质菌种资源。The aim of the study was to isolate lactic acid bacteria with the ability to alleviate hyperuricemia from Tibetan kefir grains.Based on high-performance liquid chromatography(HPLC),the degradation ability of inosine,guanosine,and adenosine was investigated in 28 selected strains.Among them,Lactobacillus ZD103 had the highest ability to degrade inosine,guanosine,and adenosine.To further analyse the potential uric acid-lowering effect of strain ZD103,the inhibition of xanthine oxidase(XOD)by metabolites of strain ZD103 was determined by the HPLC method.Results showed that metabolites from strain ZD103 significantly inhibited XOD activity and that the inhibitory effect was exerted by acids present in the extracellular metabolites.Based on molecular docking,the acids in the metabolites were docked to XOD and the three substances with the highest scores were selected to test their inhibitory effects on XOD.Results showed that the three substances,1-hydroxy-2-naphthylcarboxylic acid,3-indoleacrylic acid,and indolepyruvic acid,all had different degrees of inhibitory effects on XOD,among which 1-hydroxy-2-naphthylcarboxylic acid had the best inhibitory effect,with the inhibition rate of XOD reaching(98.66±1.63)%.This study provides a theoretical basis and high-quality strain resources for the development of probiotic products for the prevention and treatment of hyperuricemia.

关 键 词：高尿酸血症 核苷 乳酸菌 黄嘌呤氧化酶 分子对接 

分 类 号：TS201.3[轻工技术与工程—食品科学]