响应面法优化金鲳鱼皮抗氧化肽制备工艺及其组成分析  

Optimization of Preparation Process of Antioxidant Peptides from Golden Pompano(Trachinotus ovatus)Skin by Response Surface Method and Its Composition Analysis

作　　者：苏梓盈 陆旭丽 代亚萍 曹玉坡[2] 郑龙[2] 李积华[2] 张怡[1] SU Ziying;LU Xuli;DAI Yaping;CAO Yupo;ZHENG Long;LI Jihua;ZHANG Yi(College of Food Science,Fujian Agricultural and Forestry University,Fuzhou 350002,China;Key Laboratory of Tropical Crop Products Processing,Ministry of Agriculture and Rural Affairs,Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524001,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]中国热带农业科学院农产品加工研究所,农业农村部热带作物产品加工重点实验室,广东湛江524001

出　　处：《食品工业科技》2024年第23期198-207,共10页Science and Technology of Food Industry

基　　金：海南省自然科学基金项目(320MS092,322QN382);中央级公益性科研院所基本科研业务费专项(1630122022004);湛江市科技计划项目(2021A05029)。

摘　　要：为促进金鲳鱼皮资源的综合利用,本研究以金鲳鱼皮为原料,探究其抗氧化肽的最佳制备工艺。以水解度和DPPH自由基清除率为考察指标,在单因素实验的基础上,结合响应面试验对金鲳鱼皮的酶解条件包括蛋白酶种类、酶解时间、加酶量、酶解温度以及酶解pH进行优化,得到金鲳鱼皮抗氧化肽的最佳酶解条件,并测定最佳条件下得到的酶解产物的分子量分布及氨基酸组成。结果表明,制备金鲳鱼皮抗氧化肽酶解效果最优的酶为碱性蛋白酶,其最佳酶解工艺为:酶解温度52.3℃、酶解pH10.0、酶解时间8.4 h、加酶量15409 U/g。通过最佳酶解条件制备得到的酶解产物的DPPH自由基清除率为81.59%±2.96%,其分子量主要分布在3000 Da以下,占比高达79.20%,其含有17种氨基酸,甘氨酸为主要氨基酸,疏水性氨基酸占比35.28%。本研究结果为金鲳鱼皮的高值化利用与抗氧化肽的筛选提供了一定的理论依据。In order to promote the comprehensive utilization of the resource of golden pompano skin,this study investigated the optimal preparation process of its antioxidant peptides using golden pompano skin as raw material.Taking hydrolysis degree and DPPH free radical scavenging rate as inspection indexes,the enzymatic hydrolysis conditions of golden pompano skin,including enzyme type,time,enzyme amount,temperature and pH were optimized on the basis of single factor experiment combined with response surface methodology.Therefore,the optimal enzymatic hydrolysis conditions of antioxidant peptides from golden pompano skin were obtained,and the molecular weight distribution and amino acid composition of enzymolysis product were further determined.The results showed that the optimal enzyme for the preparation of antioxidant peptides from the golden pompano skin was alkaline protease,and the optimal enzymatic hydrolysis conditions were:Enzymolysis temperature 52.3℃,pH10.0,time 8.4 h and enzyme amount 15409 U/g.The DPPH free radical scavenging rate of the enzymolysis product obtained under the optimal enzymatic hydrolysis conditions was 81.59%±2.96%.The molecular weight of the enzymolysis product was mainly distributed below 3000 Da,accounting for 79.20%.Besides,17 kinds of amino acids were detected in the enzymolysis product,of which glycine was the main amino acid and hydrophobic amino acids accounted for 35.28%.The results of this study provide a theoretical basis for the high-value utilization of golden pompano skin and the screening of antioxidant peptides.

关 键 词：金鲳鱼皮 抗氧化肽 酶解工艺优化 分子量分布 氨基酸组成 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]