NaCl胁迫对植物乳植杆菌IMAUJBP3抗氧化能力的影响及转录组分析  

Effect of NaCl Stress on Antioxidant Capacity of Lactiplantibacillus plantarum IMAUJBP3 and Transcriptome Analysis

作　　者：王怡 韩军 孙芮君 田建军[1,2] 靳烨 WANG Yi;HAN Jun;SUN Ruijun;TIAN Jianjun;JIN Ye(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;Integrative Research Base of Beef and Lamb Processing Technology,Ministry of Agriculture and Rural Affairs of the People's Republic of China,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]农业农村部生鲜牛羊肉加工技术集成基地,内蒙古呼和浩特010018

出　　处：《食品工业科技》2024年第24期167-177,共11页Science and Technology of Food Industry

基　　金：内蒙古自治区科技计划项目(2022YFHH0112);阿拉善盟科技计划项目(AMKJ2023-04);“科技兴蒙”行动重点专项巴彦淖尔国家农业高新技术产业示范区重点项目(NMKJXM202205);呼和浩特市高校院所协同创新项目(XTCX2023-18)。

摘　　要：为探究植物乳植杆菌IMAIJIBP3在6.5%NaCl胁迫处理下菌株抗氧化性能的变化,对其抗氧化表观指标和转录组学进行探究。结果表明,IMAUJBP3经NaCl处理后的菌悬液、发酵上清液、胞内提取物的DPPH自由基、羟基自由基的清除率均高于对照组;但对于超氧阴离子自由基,NaCl处理后的菌悬液和发酵上清液的清除率高于对照组,而胞内提取物清除超氧阴离子自由基的能力却显著(P<0.05)低于对照组,在NaCl处理后,菌株的总抗氧化能力下降。转录组分析表明,氧化还原相关基因中谷胱甘肽-谷胱甘肽还原酶系统和硫氧还蛋白-硫氧还蛋白还原酶系统中的关键基因表达量呈上调趋势,与能量相关基因表达多呈下调趋势。JBP3主要通过转录、碳水化合物转运和代谢适应NaCl胁迫,通过调控细胞和代谢的生物过程,调控细胞实体结构,影响催化活性和rRNA与蛋白质的结合位点,进而缓解NaCl胁迫处理的不良反应,同时能够通过调控阴离子外排,缓解胞内的渗透压力。因此,植物乳植杆菌IMAUJBP3具备调控盐胁迫处理下的抗氧化功能,具有实际应用的潜力。In order to explore the changes of antioxidant performance of Lactiplantibacillus plantarum IMAIJIBP3 strain under 6.5%NaCl stress,antioxidant apparent indexes and transcriptomics of IMAUJBP3 strain were investigated.The results showed that the scavenging rates of DPPH radical and hydroxyl radical of bacterial suspension,fermentation supernatant and intracellular extract of IMAUJBP3 treated by NaCl were higher than those of the control group.However,in terms of superoxide anion clearance,the scavenging rate of bacterial suspension and fermentation supernatant treated with NaCl was higher than that of the control group,while the superoxide anion scavenging ability of intracellular extracts was significantly(P<0.05)lower than that of the control group.After NaCl treatment,the total antioxidant capacity of the strain decreased.Transcriptome analysis showed that the redox-related genes were up-regulated in glutathione-glutathione reductase system and thioredoxin-thioredoxin reductase system,while energy-related genes were mostly down-regulated the most.JBP3 adapts to NaCl stress mainly through transcription and carbohydrate transport and metabolism,regulates cell physical structure by regulating the biological process of cell and metabolism,affects catalytic activity and the binding site between rRNA and protein,and alleviates the adverse reactions of NaCl stress treatment.At the same time,it can relieve intracellular osmotic pressure by regulating anion efflux.Therefore,Lactiplantibacillus plantarum IMAUJBP3 had the function of regulating antioxidation under NaCl stress and had the potential of practical application.

关 键 词：植物乳植杆菌 NACL 胁迫 抗氧化能力 转录组分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]