不同提取方法对密银花多糖理化性质及抗氧化活性的影响  

Effects of Different Extraction Methods on Physicochemical Properties and Antioxidant Activity of Polysaccharides from Lonicerae japonicae Flos

作　　者：王国霞 牛祎琳 牛富婷 程莹莹 WANG Guoxia;NIU Yilin;NIU Futing;CHENG Yingying(School of Life Sciences,Zhengzhou Normal University,Zhengzhou 450044,China)

机构地区：[1]郑州师范学院生命科学学院,河南郑州450044

出　　处：《食品工业科技》2024年第24期196-203,共8页Science and Technology of Food Industry

基　　金：河南省高等学校重点科研项目计划(24B550024)。

摘　　要：本文以密银花为原料,比较热水浸提法(Hot Water Extraction,HWE)、酶解法(Enzyme Extraction,EE)、超声波辅助法(Ultrasonic-assisted Extraction,UE)和超声波辅助酶法(Ultrasonic-Enzyme Extraction,UEE)对密银花多糖理化性质及抗氧化活性的影响。结果表明:四种方法提取的密银花多糖得率从高到低依次为UE-LJP(7.74%)、EE-LJP(4.35%)、UEE-LJP(3.38%)、HWE-LJP(2.13%)。不同提取方法所得的密银花多糖中均含有糖醛酸,密银花多糖是一种酸性多糖。超声波辅助提取密银花多糖中蛋白质含量最低(0.16%)、持水性及水合特性最强;酶法提取的密银花多糖中总糖含量较高(38.68%)。抗氧化活性表明,四种密银花多糖均具有较强的抗氧化活性。其中,HWE-LJP、UE-LJP和EE-LJP具有较强的DPPH自由基清除能力,半抑制浓度分别为0.067、0.104、0.093 mg/mL,在羟基自由基方面,UEE-LJP表现出较强的清除能力,半抑制浓度为0.390 mg/mL。因此,不同提取方法对密银花多糖的理化性质和抗氧化活性具有显著的影响,UE法和EE法可作为提取密银花多糖最适宜的方法,本文将为开发功能性食品和抗氧化等功能药物原料的研发提供参考。In this paper,honeysuckle was used as raw material to compare the effects of hot water extraction(HWE),enzyme extraction(EE),ultrasonic-assisted extraction(UE)and ultrasonic-enzyme extraction(UEE)on the physicochemical properties and antioxidant activity of honeysuckle polysaccharides.The results showed that the yield of polysaccharide extracted by the four methods from high to low was UE-LJP(7.74%),EE-LJP(4.35%),UEE-LJP(3.38%)and HWE-LJP(2.13%).The polysaccharides obtained by different extraction methods all contain uronic acid,and the polysaccharide was an acidic polysaccharide.The ultrasonic-assisted extraction of polysaccharide from Lonicerae japonicae Flos had the lowest protein content(0.16%),the strongest water holding capacity and hydration characteristics.The total sugar content of polysaccharide extracted by enzymatic method was higher(38.68%).The antioxidant activity showed that the four polysaccharides had strong antioxidant activity.Among them,HWE-LJP,UE-LJP and EE-LJP had strong DPPH free radical scavenging ability,and the half inhibitory concentration was 0.067,0.104 and 0.093 mg/mL,respectively.In terms of hydroxyl radicals,UEE-LJP showed strong scavenging ability,and the half inhibitory concentration was 0.390 mg/mL.Therefore,different extraction methods had significant effects on the physical and chemical properties and antioxidant activity of Lonicerae japonicae Flos polysaccharides.UE method and EE method could be used as the most suitable methods for extracting Lonicerae japonicae Flos polysaccharides.This paper would provide reference for the development of functional food and antioxidant and other functional drug raw materials.

关 键 词：密银花 多糖 提取方法 抗氧化 热水浸提法 酶解法 超声波辅助法 

分 类 号：TS201.2[轻工技术与工程—食品科学]