沙棘果皮渣总黄酮体内外降血脂活性研究  

Hypolipidemic Activity of Total Flavonoids from Sea Buckthorn Peel Residue in Vivo and in Vitro

作　　者：孙嘉莉 赵文燕 朱东亮[2] 江峻峰 牟晓娟 闫思语 宗泽慧 包晓玮 SUN Jiali;ZHAO Wenyan;ZHU Dongliang;JIANG Junfeng;MOU Xiaojuan;YAN Siyu;ZONG Zehui;BAO Xiaowei(School of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Military Regional Security Department Drugs Instrument Supervision and Inspection Station,Urumqi 830052,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]新疆军区保障部药品仪器监督检验站,新疆乌鲁木齐830052

出　　处：《食品工业科技》2024年第24期353-363,共11页Science and Technology of Food Industry

基　　金：自治区重点研发计划项目(2022B02005-4);新疆维吾尔自治区自然科学基金面上项目(2022D01A57)。

摘　　要：为探究沙棘果皮渣总黄酮的降血脂功效及抗氧化水平,采用体外和体内试验研究沙棘果皮渣总黄酮纯化物(TFSE)降血脂功能。通过体外试验测定TFSE的抗氧化活性,并模拟人体胃肠环境,考察TFSE对三种胆酸盐的结合能力,以此来评价其体外抗氧化及降血脂效果;通过高脂饲料诱导C57BL/6J小鼠建立高脂血症模型,灌胃TFSE进行干预,分别比较不同剂量的TFSE对C57BL/6J小鼠的体重、血清和肝脏的血脂水平、肝功能指标及肝脏的氧化指标,并对肝脏组织进行病理学的观察。体外试验结果表明,TFSE对DPPH、ABTS+自由基清除能力较强,其IC50值分别5.20、10.24μg/mL,且具有较高的还原能力,说明其抗氧化活性好;与胆酸钠、甘氨胆酸钠、牛磺胆酸钠结合能力的IC50值分别为11.11、2.61、3.50 mg/mL,说明TFSE与胆酸盐的结合能力较强;体内动物实验结果表明,与模型组(HFD)相比,L-TFSE组和H-TFSE组能够抑制小鼠体重增加,显著降低(P<0.05)血清和肝脏中总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白胆固醇(LDL-C)水平,显著增加(P<0.05)高密度脂蛋白蛋白(HDL-C)水平及显著降低(P<0.05)小鼠血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)水平。同时,显著增加(P<0.05)小鼠肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)水平,显著降低(P<0.05)丙二醛(MDA)含量。综上,TFSE可以改善高脂饮食对肝脏的病理损伤,并显现出良好的降血脂活性以及抗氧化活性,该研究可为提高新疆沙棘果皮渣产品的附加值提供一定的理论基础。To investigate the hypolipidemic effects and antioxidant levels of total flavonoids from seabuckthorn fruit peel and residue,in vitro and in vivo experiments were conducted to examine the hypolipidemic functionality of the purified total flavonoids from seabuckthorn fruit peel and residue(TFSE).The antioxidant activity of TFSE was determined through in vitro experiments,and its binding capacity to three types of bile acids was investigated by simulating the human gastrointestinal environment.This was conducted to evaluate its antioxidant and hypolipidemic effects in vitro.In the in vivo experiment,a high-fat diet-induced hyperlipidemia model was established in C57BL/6J mice,and TFSE was administered orally to compare its effects on body weight,serum,hepatic lipid levels,liver function indicators,and oxidative indicators.Then,histopathological observations of the liver tissue were completed.The in vitro study showed that TFSE exhibited strong scavenging abilities against DPPH and ABTS+radicals,with IC50 values of 5.20 and 10.24μg/mL,respectively,thus indicating effective antioxidant activity.Moreover,TFSE showed strong binding capabilities with sodium cholate,glycocholate,and taurocholate,with IC50 values of 11.11,2.61,and 3.50 mg/mL,respectively,thus highlighting its binding solid capacity with bile acids.Results of the mice experiments showed that,compared to the model group(HFD),the L-TFSE and H-TFSE groups showed inhibited body weight gain,whilst(P<0.05)total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)levels in serum and liver were significantly decreased.At the same time,significantly increased(P<0.05)high-density lipoprotein cholesterol(HDL-C)levels and significantly decreased(P<0.05)levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(AKP),and lactate dehydrogenase(LDH)in mice serum.Furthermore,TFSE significantly increased(P<0.05)the superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and catalase(CAT)levels in mice liver,and

关 键 词：沙棘果皮渣 总黄酮 抗氧化活性 胆酸盐结合能力 降血脂活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]