河套地区中高温大曲真菌类群结构解析  

Analysis of fungal community structure of medium-high temperature Daqu in Hetao area

作　　者：李娜 张海波[1,2] 叶明波 张敏 李擎 郭壮[1,2,3,4] 王玉荣 LI Na;ZHANG Haibo;YE Mingbo;ZHANG Min;LI Qing;GUO Zhuang;WANG Yurong(Brewing Technology Industrial College,Hubei University of Arts and Sciences,Xiangyang 441053,China;Hubei ProvincialEngineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;Xiangyang Maotai-Flavor Baijiu Ecological Brewing Enterprise-University Joint Innovation Center,Xiangyang 441053,China;Hubei Nanzhang Sorghum Deep Processing Industry Science and Technology Backyard,Xiangyang 441053,China;Inner Mongolia Hetao Wine Group Co.,Ltd.,Bayannur 015400,China)

机构地区：[1]湖北文理学院酿酒技术产业学院,湖北襄阳441053 [2]湖北文理学院湖北省食品配料工程技术研究中心,湖北襄阳441053 [3]襄阳市酱香型白酒生态酿造企校联合创新中心,湖北襄阳441053 [4]湖北南漳高粱深加工产业科技小院,湖北襄阳441053 [5]内蒙古河套酒业集团股份有限公司,内蒙古巴彦淖尔015400

出　　处：《中国酿造》2024年第12期110-115,共6页China Brewing

基　　金：湖北省自然科学基金计划襄阳创新发展联合基金项目(2023AFD049);湖北文理学院教师科研能力培育基金“科技创新团队”(2020kypytd009);湖北文理学院科研启动基金(kyqdf2021014)。

摘　　要：该研究基于Illumina Mi Seq高通量测序技术解析内蒙古巴彦淖尔河套地区中高温大曲真菌菌群多样性及群落结构,并与江西鹰潭地区中高温大曲真菌菌群进行比较分析,解析其真菌菌群特征。结果表明,河套地区中高温大曲样品中真菌门以子囊菌门(Ascomycota)为主,真菌属以嗜热真菌属(Thermomyces)、嗜热子囊菌属(Thermoascus)、曲霉菌属(Aspergillus)和毕赤酵母属(Pichia)为主;通过多样性对比发现,与鹰潭地区相比,河套地区中高温大曲样品的真菌菌群丰富度和多样性均显著偏低(P<0.05);通过对比关键真菌属发现,河套地区中高温大曲样品含有更丰富的Thermoascus和Thermomyces,而根霉菌属(Rhizopus)、Aspergillus和横梗霉属(Lichtheimia)的相对丰度较小;通过对比相关性结果发现,2个地区中高温大曲样品的真菌属之间相关性存在较大的差异,其中河套地区中高温大曲样品中真菌属间关系更密切且稳定性更高。由此可见,河套地区中高温大曲含有丰富的以Thermomyces、Thermoascus、Aspergillus和Pichia等为主的真菌类群,且不同地区生产的中高温大曲真菌群落结构存在一定差异。The fungal flora diversity and community structure of the medium-high temperature Daqu in Hetao area of Bayannur,Inner Mongolia was analyzed by Illumina MiSeq high-throughput sequencing technology,and compared with the fungal flora of medium-high temperature Daqu in Yingtan area of Jiangxi,and the characteristics of fungal flora were analyzed.The results showed that the fungal phyla in the medium-high temperature Daqu samples in Hetao area were mainly Ascomycota,while the fungal genera were Thermomyces,Thermoascus,Aspergillus,and Pichia.The diversity comparison showed that compared with the Yingtan area,the richness and diversity of fungal flora in the medium-high temperature Daqu samples in Hetao area were significantly lower(P<0.05).By comparing key fungal genera,it was found that the Thermoascus and Thermomyces were more abundant in medium-high temperature Daqu samples in Hetao area,while the relative abundance of Rhizopus,Aspergillus and Lichtheimia was less.By comparing correlation analysis results,it was found that there were significant differences in the correlation between fungi genera in the samples from the two regions,among them,the relationship between fungi genera was closer and more stable in the samples from the middle and high temperature Daqu in Hetao area.In conclusion,Thermomyces,Thermoascus,Aspergillus and Pichia were abundant in medium-high temperature Daqu in Hetao region,and there were some differences in the fungal community structure of medium-high temperature Daqu produced in different regions.

关 键 词：河套地区 中高温大曲 高通量测序 真菌菌群多样性 相关性分析 

分 类 号：TS261.1[轻工技术与工程—发酵工程]