酸法和酶法提取对中华草龟皮胶原蛋白结构和理化特性的影响  

Effects of Acid and Enzymatic Extraction on the Structure and Physicochemical Characterization of Collagen from Chinemys reevesii Skin

作　　者：虞丹丹 魏文志[3] 关利 陈文卓 曹晖[1,2] YU Dandan;WEI Wenzhi;GUAN Li;CHEN Wenzhuo;CAO Hui(College of Tourism and Culinary Science,Yangzhou University,Yangzhou 225127,China;Key Laboratory of Chinese Cuisine Intangible Cultural Heritage Technology Inheritance,Ministry of Culture and Tourism,Yangzhou University,Yangzhou 225127,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225001,China)

机构地区：[1]扬州大学旅游烹饪学院,江苏扬州225127 [2]扬州大学中餐非遗技艺传承文化和旅游部重点实验室,江苏扬州225127 [3]扬州大学动物科学与技术学院,江苏扬州225001

出　　处：《食品工业科技》2025年第1期79-86,共8页Science and Technology of Food Industry

基　　金：苏北科技专项(2021022)。

摘　　要：本研究以中华草龟皮胶原蛋白为研究对象,探究不同提取方法对其结构和理化特性的影响。分别采用酸法和酶法对中华草龟皮胶原蛋白进行提取,并运用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、高效液相色谱法、圆二色光谱法、差式扫描量热、扫描电镜、溶解性、感官评价及色度等分析酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC)的组成、结构以及理化特性。结果表明,ASC和PSC的提取率分别为24.50%±0.44%和51.68%±0.06%,酶法提取胶原蛋白的提取率显著高于酸法(P<0.05)。ASC和PSC主要由α1和α2链组成,均为I型胶原蛋白。ASC和PSC中甘氨酸的含量最多,分别是370.17和387.75个残基/1000个氨基酸残基。圆二色光谱表明这两种胶原蛋白二级结构相似,均具有胶原蛋白的特征峰。ASC和PSC的变性温度分别为27.21±0.28℃和28.37±0.23℃。扫描电镜结果表明,ASC具有无序的纤维结构,而PSC的微观结构更为规则。ASC的溶解度整体略低于PSC,PSC的感官品质和色度优于ASC。This study investigated the effects of different extraction methods on the structure and physicochemical characterization of collagen extracted from the skin of Chinemys reevesii.Collagen from Chinemys reevesii skin was obtained using acid and enzymatic extraction methods.The composition,structure,and physicochemical characteristics of acid-soluble collagen(ASC)and enzyme-soluble collagen(PSC)were determined using sodium dodecyl sulfate�polyacrylamide gel electrophoresis(SDS-PAGE),high-performance liquid chromatography(HPLC),circular dichroism spectrometry(CD),differential scanning calorimetry(DSC),scanning electron microscopy(SEM),solubility,sensory evaluation,and chroma.ASC and PSC extraction yields were 24.50%±0.44%and 51.68%±0.06%respectively,with PSC demonstrating significantly higher extraction yield than ASC(P<0.05).Both ASC and PSC comprisedα1 andα2 chains and exhibited a distribution pattern typical of typeⅠcollagen.The content of glycine in ASC and PSC was the highest,370.17 and 387.75 residues/1000 amino acid residues,respectively.ASC and PSC exhibited similar secondary structures,according to their comparable characteristic absorption peaks.The thermal denaturation temperatures of ASC and PSC were 27.21±0.28℃and 28.37±0.23℃,respectively.SEM images indicated a disordered fiber state in the microstructure of ASC,whereas the microstructure of PSC exhibited a more uniform pattern.The solubility of ASC was slightly lower than that of PSC,and PSC had better sensory evaluation and chroma than ASC.

关 键 词：中华草龟皮 胶原蛋白 酸法提取 酶法提取 结构 理化特性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]