苯乳酸对霍氏肠杆菌及其生物被膜的抑制作用  

Inhibitory Effects of Phenyllactic Acid on Enterobacter hormaechei and Its Biofilm

作　　者：孙家兴 李秋莹 崔方超 檀茜倩 孙彤 Sun Jiaxing;Li Qiuying;Cui Fangchao;Tan Xiqian;Sun Tong(College of Food Science and Engineering,Bohai University,Jinzhou 121013,Liaoning;National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,Liaoning)

机构地区：[1]渤海大学食品科学与工程学院,辽宁锦州121013 [2]生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013

出　　处：《中国食品学报》2024年第11期40-49,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(32202141)。

摘　　要：目的:探究苯乳酸(PLA)对霍氏肠杆菌及其生物被膜中的抑制作用。方法:通过最小抑菌浓度(MIC)、最小杀菌浓度(MBC)、生长曲线、流式细胞术、碱性磷酸酶(AKP)活性、细胞膜完整性、DNA含量、运动能力、生物被膜形成量的测定与激光共聚焦观察等试验,评价PLA对霍氏肠杆菌及其生物被膜的抑制作用。结果:PLA对霍氏肠杆菌的MIC和MBC分别为1.25 mg/mL和2.5 mg/mL,在该质量浓度下可完全抑制霍氏肠杆菌生长。流式细胞仪检测结果表明:1/2 MIC和MIC苯乳酸不会导致细胞凋亡和坏死,而2 MIC PLA可造成细胞死亡。28℃培养下霍氏肠杆菌的生长曲线虽较37℃培养延滞近1 h,但未影响其最大生物量,且细胞凋亡率没有显著差异。PLA破坏了细胞结构的完整性,造成细胞内AKP活性降低,核酸与蛋白质泄漏;2 MIC PLA可导致菌体DNA含量显著降低(P<0.05);PLA处理可导致霍氏肠杆菌运动能力和生物被膜生成量降低。结论:PLA对霍氏肠杆菌及其生物被膜有较强的抑制作用。Objective:The inhibitory effects of phenyllactic acid on Enterobacter hormaechei and its biofilm was explored.Method:The inhibitory effect of PLA on E.hormaechei and its biofilm was assessed through various experimental techniques,including measurement of minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC),growth curve,flow cytometry,alkaline phosphatase(AKP)activity,cell membrane integrity,DNA content,motility test,biofilm formation and confocal laser scanning microscopy observation.Results:The MIC and MBC of PLA to E.hormaechei was 1.25 mg/mL and 2.5 mg/mL,respectively,which could completely inhibit the growth of E.hormaechei.Flow cytometry results showed that 1/2 MIC and MIC phenyllactic acid could not cause apoptosis and necrosis,while 2 MIC PLA could cause cell death.The lag phase of growth curve of E.hormaechei cultured at 28℃was nearly 1 h longer than that at 37℃,but the maximum biomass of E.hormaechei was not affected,and the apoptosis rate was not significantly different.PLA damaged the structural integrity of cells,resulting in decreased intracellular AKP activity and leakage of nucleic acids and proteins.2 MIC PLA could significantly reduce the content of bacterial DNA.PLA treatment resulted in decreased motor capacity and biofilm production of E.hormaechei.Conclusion:PLA had a strong inhibitory effect on E.hormaechei and its biofilm.

关 键 词：苯乳酸 霍氏肠杆菌 抑菌 生物被膜 

分 类 号：TS201.3[轻工技术与工程—食品科学]