Metabolite profiling and Ameliorative effect of quince(Cydonia oblonga)leaves against doxorubicin induced cardiotoxicity in Wistar rats  

作　　者：Entesar Hanan Nazeer Hasan Sultan Zahiruddin Sayeed Ahmad Vasudha Sharma Farhan J.Ahmad 

机构地区：[1]Department of Food Technology,School of Interdisciplinary Science and Technology,Jamia Hamdard,New Delhi,110062,India [2]Department of Pharmaceutics,School of Pharmaceutical Education and Research,Jamia Hamdard,New Delhi,110062,India [3]Department of Pharmacognosy and Phytochemistry,School of Pharmaceutical Education&Research,Jamia Hamdard,New Delhi,110062,India

出　　处：《Food Bioscience》2023年第3期1813-1825,共13页食品生物科学（英文）

基　　金：Department of Science and Technology(DST),New Delhi,India,for providing the fellowship to Entesar Hanan(DST/INSPIRE Fellowship/IF170403)。

摘　　要：Quince(Cydonia oblonga)leaves are traditionally reported to alleviate the risk of cardiovascular disease.The study envisaged to explore the in vitro and in vivo phytochemical and cardio protective potential of quince extract respectively.Different solvent extracts(Aqueous,Ethanolic,Hydroethanolic,Methanolic)of quince leaf obtained via two different extraction methods(Ultrasonication and Reflux)were evaluated for the phytochemical and antioxidant analysis.Among all the extracts,quince ethanolic extract,extracted via sonication was found to have the maximum phenolics(39.89±0.67 mg GAE/g DW),flavonoids(21.51±1.0 mgRE/g DW)and an anti-oxidant potential 2,2-diphenyl-1-picryl-hydrazyl radical(DPPH)with IC_(50) value of 168.88±2.24μg/ml.The High-performance thin layer chromatograph(HPTLC)and ultrahigh performance liquid chromatography mass spectrometry(UPLC-MS)analysis confirmed the presence of bio active compounds.Furthermore,the in vivo activity was ascertained against Doxorubicin(DOX)induced cardio toxicity in rats fed orally with the extract at 160 and 320 mg/kg body weight.The pre-treated groups significantly assuaged the changes induced by DOX in the electrocardiogram(ECG).Additionally,it mitigated the elevation in the blood serum parameters;aspartate transaminase(AST),lactate dehydrogenase(LDH),creatinine kinase-MB(CK-MB)and also repressed glutathione(GSH)depletion and rise in malondialdehyde(MDA)level induced by DOX.Furthermore,improvement in histopathological changes of the heart tissue concomitant to DOX induced toxicity was also observed such as,reduced degeneration,necrosis,inflammation and apoptosis.Thus,it could be concluded that cardioprotective activity of quince extract accredited to the occurrence of phytoconstituents prevented the DOX induced cardiotoxicity and helped to reinstate the cardiac damage in rats.

关 键 词：QUINCE DOXORUBICIN Cardio protection METABOLITES ANTI-OXIDANTS 

分 类 号：TS201.4[轻工技术与工程—食品科学]