Eucalyptol prevents pilocarpine-induced seizure and neuronal damage in mice,through the cholinergic,monoaminergic and antioxidant pathways  

作　　者：Daniel Souza Bezerra Gyllyandeson de Araujo Delmondes Maria Janice Pereira Lopes Isaac Moura Araujo Giovana Mendes de Lacerda Leite Maysa de Oliveira Barbosa Roseli Barbosa Adriano Francisco Alves Cassio Rocha Medeiros Sloana Giesta Lemos Florencio Irwin Rose Alencar de Menezes Henrique Douglas Melo Coutinho Cicero Francisco Bezerra Felipe Marta Regina Kerntopf 

机构地区：[1]Regional University of Cariri(URCA),Department of Biological Chemistry(DBQ),Pimenta,Crato,63105-000,Ceará-CE,Brazil [2]Federal University of Paraíba(UFPB),Departamento de Biologia Molecular(DBM),Cidade Universitária,58051-900,João Pessoa,PB,Brazil [3]CECAPE College,Av.Padre Cícero,3917,São José,Juazeiro do Norte,CE,63024-015,Brazil

出　　处：《Food Bioscience》2023年第3期3059-3066,共8页食品生物科学（英文）

基　　金：supported by the Cearense Foundation for Support to Scientific(Fundaçāo Cearense de Apoio ao Desenvolvimento Científico e Tecnológico)[grant number:BMD-0008-01254.01.16/18,2018];the Regional University of Cariri.

摘　　要：Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has been stimulating the prospection of new molecules isolated from aromatic plants,with potential anticonvulsant and neuroprotective activities and less side effects.This study aimed to evaluate,though behavioural and neuro-chemical methodologies,the anticonvulsant,and neuroprotective effects of eucalyptol on mice subjected to the pilocarpine-induced seizure model.Eucalyptol(100,200 and 400 mg/kg,p.o.)was administered in mice prior to pilocarpine(350 mg/kg,i.p.)and the following behavioural parameters were assessed:Latency to First Seizure(LFS),Seizure Intensity(SI)and Latency to Death(LD).In addition,an oxotremorine-induced tremors test was performed to evaluate the cholinergic system involvement on the eucalyptol effects.Neurochemical tests were also performed,including determination of hippocampal(HC)concentration of thiobarbituric acid reactive substances(TBARS)and nitrite/nitrate and striatal(ST)concentration of noradrenaline,dopamine,and sero-tonin.Lastly,histopathological,and morphometric hippocampal analysis were conducted.Eucalyptol increased the latency to first seizure and latency to death,inhibited oxotremorine-induced tremors,decreased hippocampal TBARS and nitrite/nitrate overproduction,increased striatal noradrenaline and dopamine levels and prevented hippocampal neurodegeneration.These results demonstrate the potential anticonvulsant,neuroprotective and antioxidant effects of eucalyptol,probably through a conjunction of mechanisms including muscarinic cholin-ergic antagonism,oxidative stress mitigation and the monoaminergic system modulation,which appears to effectively control the seizure onset.

关 键 词：SEIZURE PILOCARPINE EUCALYPTOL MONOAMINES ANTIOXIDANT Neuroprotection 

分 类 号：TS201.4[轻工技术与工程—食品科学] R96[轻工技术与工程—食品科学与工程]