覆盆子总黄酮的酶辅助闪式提取及其抗氧化、降血糖和降血脂活性分析  

Enzyme-assisted flash extraction of total flavonoids from raspberry and analysis of its antioxidant,hypoglycemic,and hypolipidemic activities

作　　者：张琪婧 耿振甲 李敏 王俊秋 钱思颖 师聪 ZHANG Qi-jing;GENG Zhen-jia;LI Min;WANG Jun-qiu;QIAN Si-ying;SHI Cong(School of Food and Biological Engineering,Xuzhou University of Technology,Xuzhou 221018,Jiangsu,China)

机构地区：[1]徐州工程学院食品与生物工程学院,江苏徐州221018

出　　处：《粮食与油脂》2025年第1期115-120,126,共7页Cereals & Oils

基　　金：国家自然科学基金青年项目(32102121);江苏省自然科学基金青年项目(BK20210078);江苏省产学研合作项目(BY20231339)。

摘　　要：采用酶辅助闪式提取覆盆子中总黄酮。在单因素试验基础上采用响应面法优化其提取工艺条件,同时对提取的覆盆子总黄酮进行体外抗氧化、降血糖和降血脂活性研究。结果表明:覆盆子总黄酮最佳提取工艺条件为酶质量浓度2mg/mL、酶解温度46℃、提取次数3、液固比36:1(mL/g),在此条件下覆盆子总黄酮得率为1.40mg/g。体外试验结果表明,覆盆子总黄酮具有一定的抗氧化、降血糖和降血脂能力。Total flavonoids were extracted from raspberry by enzyme-assisted flash.The extraction condi-tions were optimized by response surface methodology on the basis of single factor experiments,and their antioxidant,hypoglycemic and hypolipidemic activity in vitro were studied.The results showed that the optimal conditions for the extraction of total flavonoids from raspberry were enzyme mass concentration 2 mg/mL,enzymatic hydrolysis temperature 46℃,extraction times3,liquid-material ratio 36:1(mL/g).Under these conditions,the yield of total flavonoids from raspberry was 1.40 mg/g.The re-sults of in vitro experiments showed that total flavonoids from raspberry had certain antioxidant,hypogly-cemic,and hypolipidemic abilities.

关 键 词：覆盆子 总黄酮 抗氧化活性 降血糖 降血脂 

分 类 号：TS201.2[轻工技术与工程—食品科学]