驼乳中牛乳掺假快速检测双重胶体金免疫层析试纸条的研制  

Development of Duplex Colloidal Gold Immunochromatographic Test Strips for Rapid Detection of Cow's Milk Adulteration in Camel Milk

作　　者：庞祥艺 张娟[1] 王楠 李晓雨 陈嘉词 樊霞[1] 郑文新 陈爱亮[1] PANG Xiangyi;ZHANG Juan;WANG Nan;LI Xiaoyu;CHEN Jiaci;FAN Xia;ZHENG Wenxin;CHEN Ailiang(Institute of Quality Standard and Testing Technology for Agro-Products of CAAS,Beijing 100081,China;Institute of Animal Husbandry Quality Standard,Xinjiang Academy of Animal Science,Urumqi 83001l,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京100081 [2]新疆畜牧科学院畜牧业质量标准研究所,新疆乌鲁木齐830011

出　　处：《食品科技》2024年第12期281-287,共7页Food Science and Technology

基　　金：国家重点研发计划项目(2023YFF1104701-03)。

摘　　要：驼乳中掺假牛乳现象逐渐增多,而现有的检测驼乳中牛乳掺假的方法较费时费力。该研究的目的是建立一种快速、简便、灵敏且特异的方法,用于鉴别驼乳中是否掺有牛乳。该方法使用胶体金纳米颗粒标记抗骆驼IgG、牛IgG的特异性抗体,开发了一种基于竞争法的双重胶体金免疫层析试纸条,用于检测样品中的骆驼IgG和牛IgG,根据试纸条检测线(T)与质控线(C)的显色结果,判断样品中是否含有驼乳和牛乳成分。该方法可在10~15 min内读取结果,牛乳检出限为5%,操作简便,适宜现场快速检测。Adulteration of cow’s milk with camel’s milk is gradually increasing and the existing methods for detecting cow’s milk adulteration in camel’s milk are time-consuming and laborious.The objective of this study was to develop a rapid,simple,sensitive and specific method for detecting the presence of cow’s milk in camel’s milk.The method uses colloidal gold nanoparticles labeled with specific antibodies against camel IgG and bovine IgG,and a competitive method of duplex colloidal gold immunochromatographic test strip was developed for detecting camel IgG and bovine IgG in samples.The presence of camel milk and cow’s milk components in the samples is determined based on the color development of the test line(T)and the control line(C)on the test strip.The results can be read within 10–15 min,and the detection limit of cow’s milk is 5%.This method is easy to operate and suitable for rapid detection in the field.

关 键 词：驼乳 牛乳 掺假 IGG 免疫层析试纸条 

分 类 号：TS207.3[轻工技术与工程—食品科学]