甘草渣发酵产非淀粉多糖酶菌株筛选及产酶的动态变化  

Screening of strains producing non-starch polysaccharide enzyme by fermentation of licorice residue and its dynamic changes

作　　者：杨奕凡 高雁[2] 刘亚男 霍向东[2] 娄恺[2] 关波[3] 陈开旭 曾军[2] YANG Yifan;GAO Yan;LIU Yanan;HUO Xiangdong;LOU Kai;GUAN Bo;CHEN Kaixu;ZENG Jun(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Microbial Applications,Xinjiang Academy of Agricultural Sciences/Xinjiang Special Environment Microbial Laboratory,Urumqi 830091,China;College of Food Science,Shihezi University,Shihezi Xinjiang 832000,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091 [3]石河子大学食品学院,新疆石河子830091

出　　处：《新疆农业科学》2024年第11期2733-2741,共9页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发计划项目子课题(2022B02057-1);新疆维吾尔自治区乡村振兴产业发展科技行动项目(2022NC073)。

摘　　要：【目的】从4株真菌中筛选出产非淀粉多糖酶水平较强的菌株,为甘草渣固态发酵生产非淀粉多糖酶工艺奠定基础。【方法】设计刚果红法及滤纸条崩解试验,对4种真菌(黑曲霉、里氏木霉、黄孢原毛平革菌、康宁木霉)进行初筛,采用甘草渣摇瓶发酵进行复筛,测定纤维素酶(CMC酶、滤纸酶)及木聚糖酶活力。将产酶水平最高的菌株与甘草渣进行固态发酵,验证其产酶效果。【结果】4株真菌均具有产纤维素酶、木聚糖酶的能力。黑曲霉与其他3种菌株间存在拮抗作用。黄孢原毛平革菌和康宁木霉较其他2株真菌产纤维素酶较高,在发酵第4 d时CMC酶活力分别达到2.10、2.31 IU/mL。黑曲霉摇瓶复筛发酵产木聚糖酶活力最高可达97.46 IU/mL,而黄孢原毛平革菌与康宁木霉混菌发酵产木聚糖酶活力最高可达131.707 IU/mL。【结论】甘草渣与黄孢原毛平革菌、康宁木霉混合固态发酵生产出的非淀粉多糖酶活力增长周期更稳定,产量更高。甘草渣作为农业副产品有潜质成为未来生产酶的低成本底物。【Objective】To screen a strain with high yield of non-starch polysaccharide enzyme four fungi in the hope of laying a foundation for the production of non-starch polysaccharide enzyme by solid-state fermentation of licorice residue.【Methods】Four kinds of fungi(Aspergillus niger,Trichoderma reesei,Phanerochaete chrysosporium and Trichoderma koningii)were primarily screened by Congo red method and filter paper strip disintegration test,and the CMC enzyme,filter paper enzyme and xylanase activities were determined by shake flask fermentation of residue.The strain with the highest enzyme activity was fermented with licorice residue in solid state to verify its enzyme production effect.【Results】Through the preliminary screening,it was found that all four strains of fungi had the ability to produce cellulase and xylanase.The antagonistic effects between Aspergillus Niger and other three strains was found by plate confrontation test.According to the results of enzyme activity determination,Phanerochaete chrysosporium and Trichoderma koningii produced the highest cellulase than the other two fungi,and the CMC enzyme activity reached 2.10 and 2.31 IU/mL on the fourth day of fermentation.The highest xylanase activity produced by Aspergillus Niger through shake flask re-screening fermentation could reach 97.46 IU/mL.However,the xylanase activity of Phanerochaete chrysosporium and Trichoderma koningii could reach 131.707 IU/mL at the highest.【Conclusion】The growth cycle of non-starch polysaccharide activity produced by mixed solid-state fermentation of licorice residue with Phanerochaete chrysosporium and Trichoderma koningii is more stable and the yield is higher,therefore,licorice residue,as an agricultural by-product,has the potential to become a low-cost substrate for enzyme production in the future.

关 键 词：甘草渣 纤维素酶 木聚糖酶 固态发酵 

分 类 号：S182[农业科学—农业基础科学]