基于LC-MS/MS不同香型白酒、鹿茸酒及鹿血酒非挥发性代谢物差异分析  

Differences analysis of non-volatile metabolites of different flavor types Baijiu,deer antler wine and deer blood wine based on LC-MS/MS

作　　者：李林竹 苏伟 母应春 齐琦 邹恬杏 姜远平 陈乾 LI Linzhu;SU Wei;MU Yingchun;QI Qi;ZOU Tianxing;JIANG Yuanping;CHEN Qian(School of Wine Making and Food Engineering,Guizhou University,Guiyang 550025,China;Guizhou Fuluyuan Wine Industry Co.,Ltd.,Bijie 553300,China;Guizhou Xijiu Co.,Ltd.,Zunyi 564600,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州福鹿源酒业有限公司,贵州毕节553300 [3]贵州习酒股份有限公司,贵州遵义564600

出　　处：《中国酿造》2025年第2期65-72,共8页China Brewing

基　　金：国家自然科学基金地区项目(32160554);贵州省科学技术厅科服项目(黔科合服企[2020]4009(006))。

摘　　要：为全面比较清香型白酒基酒(FB)、清香型鹿茸酒(FP)、酱香型白酒基酒(MB)、酱香型鹿茸酒(MP)、酱香型鹿血酒(MA)的非挥发性代谢物差异,采用液相色谱-串联质谱(LC-MS/MS)技术鉴定并分析了五种酒之间的代谢组分差异,采用主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)构建模型,利用变量投影重要性(VIP)值及方差分析P值筛选关键差异代谢物,并进行聚类分析(CA)及京都基因与基因组百科全书(KEGG)代谢通路分析。结果表明,5种酒样共检出124种非挥发性代谢物,其中氨基酸类45种,有机酸类8种,肉桂酸衍生物4种,糖类15种,脂类18种,胺类3种,醇类2种,维生素类3种,呋喃酮类1种,苯类11种,吲哚羧酸类4种,核苷酸类1种,其他类9种。通过PCA、OPLS-DA均能有效区分五种酒。以VIP≥1和P<0.05为标准,共筛出43个关键差异代谢物。聚类分析结果显示,五种酒中MA与MP聚为一类,FB、FP和MB聚为一类。KEGG代谢通路分析表明,L-苏氨酸、L-苯丙氨酸、L-酪氨酸、L-高胱氨酸等可以作为区分五种酒的重要依据。In order to comprehensively compare the differences in non-volatile metabolites among the base liquor of the light-flavor(Qingxiangxing)Baijiu(FB),light-flavor deer antler wine(FP),sauce-flavor(Jiangxiangxing)Baijiu(MB),sauce-flavor deer antler wine(MP),and sauce-flavor deer blood wine(MA),the differences in metabolite fractions among the five alcoholic drinks were identified and analyzed using liquid chromatography-tandem mass spectrometry(LC-MS/MS).The model was constructed using principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA),and key differential metabolites were screened using the variable importance in the projections(VIP)values and P values of variance analysis,and cluster analysis(CA)and Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathway analysis were performed.The results showed that a total of 124 non-volatile metabolites were detected in the five alcoholic drinks samples,including 45 amino acids,8 organic acids,4 cinnamic acid derivatives,15 sugars,18 lipids,3 amines,2 alcohols,3 vitamins,1 furazone,11 benzenes,4 indole carboxylic acids,1 nucleotide,and 9 others.The drinks could be effectively distinguished by both PCA and OPLS-DA.A total of 43 key differential metabolites(VIP≥1 and P<0.05)were screened.By cluster analysis,MA and MP were clustered together as one group,and FB,FP and MB were clustered together as one group in the five drinks.KEGG metabolic pathway analysis showed that L-threonine,L-phenylalanine,L-tyrosine and L-homocysteine could be used as an important basis to differentiate the five drinks.

关 键 词：白酒 鹿茸酒 鹿血酒 非挥发性代谢物 差异分析 

分 类 号：TS262.8[轻工技术与工程—发酵工程]