不同地区来源浓香型白酒窖泥古菌群落结构分析  

Analysis of archaeal community structure in pit mud of strong-flavor Baijiu from different regions

作　　者：马龙 燕伟 王淑玉 林一心 尉军强 唐云 MA Long;YAN Wei;WANG Shuyu;LIN Yixin;WEI Junqiang;TANG Yun(Jinhui Liquor Co.,Ltd.,Longnan 742308,China)

机构地区：[1]金徽酒股份有限公司,甘肃陇南742308

出　　处：《中国酿造》2025年第2期185-190,共6页China Brewing

摘　　要：该研究以甘肃陇南(JH)、四川宜宾(LZ、YQ、XG)、四川成都(TC)地区浓香型白酒厂的窖泥样品为研究对象,基于高通量测序技术分析窖泥古菌菌群多样性,并采用主成分分析(PCA)和层次聚类分析(HCA)探究不同地区酒厂窖泥古菌菌群的差异性。结果表明,5种窖泥样品的操作分类单元(OTUs)分布情况类似,其中,LZ窖泥样品的古菌菌群丰富度和多样性均最高。在门水平上,5种窖泥样品中的绝对优势菌门均为广古菌门(Euryarchaeota)(相对丰度均>99%)。在属水平上,共有的优势古菌属为甲烷杆菌属(Methanobacterium)、甲烷短杆菌属(Methanobrevibacter)(相对丰度均≥10%)。此外,样品TC和JH的优势古菌属还包括甲烷八叠球菌属(Methanosarcina)(相对丰度分别为18.90%、25.18%);样品LZ的优势古菌属还包括甲烷囊菌属(Methanoculleus)(相对丰度为34.25%)和拟甲烷球菌属(Methanomethylophilus)(相对丰度为18.87%)。PCA及HCA结果表明,样品JH、TC之间及样品XG、YQ之间的古菌菌群结构相似,样品LZ的古菌菌群结构与其余窖泥样品存在较大差异。Using the pit mud samples from strong-flavor(Nongxiangxing)Baijiu factories in Longnan,Gansu(JH),Yibin,Sichuan(LZ,YQ,XG),and Chengdu,Sichuan(TC)regions as research objects,the archaeal community diversity in pit mud was analyzed by high-throughput sequencing technology,and the differences in archaeal communities of pit mud from different regions of Baijiu factories were investigated using principal component analysis(PCA)and hierarchical clustering analysis(HCA).The results showed that the distribution of operational taxonomic units(OTUs)of the 5 pit mud samples were similar,among which the LZ pit mud sample had the highest archaeal community richness and diversity.Euryarchaeota(the relative abundance>99%)was absolute dominant archaeal phylum in all 5 pit mud samples at the phylum level,and Methanobacterium and Methanobrevibacter(the relative abundance≥10%)were common dominat archeal genera at the genus level.In addition,the dominant archaeal genera also included Methanosarcina in TC and JH samples(the relative abundance were 18.90%and 25.18%,respectively),and also included Methanoculleus(the relative abundance was 34.25%)and Methanomethylophilus(the relative abundance was 18.87%)in LZ sample.The results of PCA and HCA showed that the archaeal community structure was similar between the JH and TC samples,the XG and YQ samples,and the archaeal community structure of the LZ sample was significantly different from that of other pit mud samples.

关 键 词：浓香型白酒 窖泥 古菌群落 高通量测序 层次聚类分析 

分 类 号：TS261.1[轻工技术与工程—发酵工程]