酱香型白酒丢糟总黄酮提取工艺优化及抗氧化性研究  

Optimization of extraction process and antioxidant activity of total flavonoids from sauce-flavor Baijiu distiller's grains

作　　者：李芳香 唐家乐 喻正涛 王桂圆 谢艺 LI Fangxiang;TANG Jiale;YU Zhengtao;WANG Guiyuan;XIE Yi(Department of Brewing Engineering,Moutai Institute,Zunyi 564500,China)

机构地区：[1]茅台学院酿酒工程系,贵州遵义564500

出　　处：《中国酿造》2025年第2期245-250,共6页China Brewing

基　　金：茅台学院高层次人才科研启动项目(mygccrc[2022]009);贵州省教育厅青年人才成长项目(黔教合KY字[2018]451);贵州省青年科技人才成长项目(黔教合KY字[2020]232)。

摘　　要：以贵州省仁怀市酱香型白酒副产物丢糟为研究对象,乙醇为浸提溶剂,经过水浴回流提取总黄酮。以丢糟总黄酮得率为响应值,采用单因素试验和Box-Behnken响应面试验优化提取工艺,并对其抗氧化活性进行测定。结果表明,乙醇浸提法提取丢糟总黄酮的最优工艺条件为:乙醇体积分数80%、料液比1∶23(g∶mL)、浸提时间29 min、水浴温度72℃。在此优化条件下,酱香型白酒丢糟中总黄酮的得率为4.02%。抗氧化试验结果表明,总黄酮提取液清除1,1-二苯基-2-苦肼基(DPPH)自由基和羟基自由基的半抑制浓度(IC50)值分别为18.89μg/mL和14.69μg/mL,说明酱香型白酒丢糟总黄酮提取液具有一定的抗氧化活性,可为富含黄酮配制酒的制备提供理论依据。Using the byproduct distiller's grains of sauce-flavor(Jiangxiangxing)Baijiu in Renhuai city,Guizhou province as the research object,the ethanol as the extract solvent,the total flavonoids were extracted by water bath reflux.Using total flavonoids yield from distiller's grains as the response value,the extraction process was optimized by single factor tests and Box-Behnken response surface tests,and the antioxidant activity was measured.The results showed that the optimal process conditions for the extraction of total flavonoids from the distiller's grains by ethanol extraction method were as follows:ethanol volume fraction 80%,solid and liquid ratio 1∶23(g∶ml),extraction time 29 min,and water bath temperature 72℃.Under these optimal conditions,the yield of total flavonoids in the distiller's grains of sauce-flavor Baijiu was 4.02%.The antioxidant tests results showed that the semi-inhibitory concentration(IC50)values of total flavonoids extract on 1,1-diphenyl-2-picrohydrazyl(DPPH)radicals and hydroxyl radicals were 18.89μg/ml and 14.69μg/ml,respectively,indicating that the total flavonoids extract from sauce-flavor Baijiu had certain antioxidant activity,and provided a theoretical basis for the preparation of flavone-rich blended liquor.

关 键 词：酱香型白酒 丢糟 总黄酮 乙醇浸提 提取工艺 响应面法 抗氧化性 

分 类 号：TS209[轻工技术与工程—食品科学]