不同粒径对凤凰单丛茶粉多糖提取得率的影响  

Effect of Different Particle Sizes on Extraction Yield of Tea Powder Polysaccharide from Fenghuang Dancong Tea

作　　者：丁莫 韩建荣 黄剑坚 吴丰年 林丽晓 胡蕾 DING Mo;HAN Jianrong;HUANG Jianjian;WU Fengnian;LIN Lixiao;HU Lui(College of Life Science and Food Engineering,Hanshan Normal University,Chaozhou 521041;Guangdong Key Laboratory for Functional Substances in Medicinal Edible Resources and Healthcare Products,Chaozhou 521041)

机构地区：[1]韩山师范学院生命科学与食品工程学院,潮州521041 [2]广东省粤东药食资源功能物质与治未病研究重点实验室,潮州521041

出　　处：《食品工业》2025年第1期75-80,共6页The Food Industry

基　　金：广东省粤东药食资源功能物质与治未病研究重点实验室(项目编号:2021B1212040015);韩山师范学院博士启动项目(项目编号:QD202124)。

摘　　要：以凤凰单丛茶为原料,分别采用普通机械粉碎法和超微粉碎法对原料进行粉碎处理,得到5种不同粒径的茶粉,以茶多糖提取得率为指标,探究不同粒度对茶粉多糖提取得率的影响,并对比不同粒径茶粉多糖的理化性质和体外抗氧化活性。研究表明:茶粉粒径为0.61 mm时多糖提取得率最大,可达10.21%,比普通机械粉碎的茶粉要高出4.43%;超微粉碎茶多糖流动性要明显优于普通机械粉碎,随着最大粒径减小呈现先降低后升高趋势,其膨胀性差异不明显。不同粒径茶粉多糖随浓度增加清除DPPH自由基、·OH自由基和ABTS^(+)自由基的能力均增强,随茶粉粒径减小,等浓度下茶多糖对DPPH自由基和·OH自由基清除率增强,对ABTS^(+)自由基的清除率影响较小,通过与VC阳性对照发现,不同粒径茶粉多糖的抗氧化性均低于VC溶液。Fenghuang Dancong tea was used as raw material,which was crushed by ordinary machine and superfine grinding,respectively.Five different particle sizes of tea powder were obtained.The extraction yield of tea polysaccharide was used as an indicator to explore the effect of different particle sizes on the extraction yield of tea powder polysaccharide,and the physicochemical properties and in vitro antioxidant activity of tea powder polysaccharide with different particle sizes were compared.The results showed that when the particle size of tea powder was 0.61 mm,the extraction yield of polysaccharide was the highest,reaching 10.21%,which was 4.43%higher than that of ordinary machine of tea powder.The fluidity of tea polysaccharide by superfine grinding was higher than ordinary machine.The trend of fluidity was decreased firstly and then increased with the particle size decreased.The swelling properties were not significant with the particle size decreased.The ability of tea powder polysaccharide with different particle sizes to scavenge DPPH,·OH,and ABTS^(+)radicals increased with its concentration increased.As the particle size of tea powder decreased,the scavenging rate of tea polysaccharide on DPPH and·OH radicals increased at the same concentration,while the scavenging rate of ABTS^(+)radicals was relatively small.By comparing with VC positive control,it was found that the antioxidant activity of tea powder polysaccharide with different particle sizes was lower than VC solution.

关 键 词：凤凰单丛茶 茶粉粒径 茶多糖 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]