南珠贝壳珍珠层源抗氧化肽的制备及对酪氨酸酶的抑制活性  

Preparation of Antioxidant Peptides Derived from Nacre of Nanzhu Shell and Their Inhibitory Activity against Tyrosinase

作　　者：徐嘉欣 罗哲 何安 黄潘钿 沈金鹏 郭俊斌 苗建银[1] XU Jiaxin;LUO Zhe;HE An;HUANG Pantian;SHEN Jinpeng;GUO Junbin;MIAO Jianyin(College of Food Science,South China Agricultural University,Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院广东省功能食品活性物重点实验室,广东广州510642

出　　处：《食品工业科技》2025年第6期242-251,共10页Science and Technology of Food Industry

基　　金：广西重点研发计划项目(桂科AB21220065)。

摘　　要：本研究旨在制备南珠贝壳珍珠层源抗氧化肽,评价其抗氧化和酪氨酸酶抑制活性,并对其氨基酸组成成分进行分析。以从南珠贝壳珍珠层中提取的蛋白为原料,采用DPPH自由基清除率作为评价指标,通过酶的筛选、单因素和响应面试验得到最优酶解工艺,并对其酶解物进行了氨基酸组成分析、体外抗氧化和酪氨酸酶抑制活性研究。南珠贝壳珍珠层源抗氧化肽的最优酶解工艺为:以胰蛋白酶为水解酶,酶解温度37℃、pH7.0、酶解时间3 h、酶底比0.32%,所得酶解物DPPH自由基清除率为76.34%±0.24%。在此条件下制备得到的珍珠层抗氧化肽具有优异的ABTS+自由基清除率(IC_(50)=0.1735 mg/mL)、DPPH自由基清除率(IC_(50)=2.342 mg/mL)和氧自由基清除能力(ORAC值=1124.86μmol TE/g冻干粉)。此外,珍珠层抗氧化肽还可通过混合型可逆抑制的方式抑制酪氨酸酶活力,具有良好的酪氨酸酶抑制活性,其抑制率的IC_(50)值为12.38 mg/mL。本研究结果表明珍珠层抗氧化肽在食品或化妆品领域具有良好的应用前景,为贝壳珍珠层的高值化利用提供了理论依据。The aim of this study was to prepare antioxidant peptides derived from nacre of Nanzhu shell,evaluate their antioxidant and tyrosinase inhibitory activities,and analyze their amino acid components.Using the protein from nacre of Nanzhu shell as raw material,DPPH free radical clearance rate as evaluation index,the optimal enzymatic hydrolysis process was obtained by sieve enzyme,single factor and response surface experiments.The amino acid composition,antioxidant and tyrosinase inhibitory activities of the enzymatic hydrolysis products were analyzed in vitro.The optimal enzymatic hydrolysis of the nacre of Nanzhu shell source antioxidant peptide was as follows:Using trypsin as hydrolase,hydrolysis temperature 37℃,pH7.0,enzymolysis time 3 h,enzymolysis ratio 0.32%,the DPPH free radical scavenging rate of the enzymolysis product was 76.34%±0.24%.The nacre antioxidant peptide prepared under these conditions had excellent ABTS+free radical scavenging rate(IC_(50)=0.1735 mg/mL),DPPH free radical scavenging rate(IC_(50)=2.342 mg/mL)and oxygen free radical scavenging ability(ORAC value=1124.86μmol TE/g lyophilized powder).In addition,nacre antioxidant peptides could also inhibit tyrosinase activity by mixed reversible inhibition,and had good tyrosinase inhibitory activity,the inhibitory rate of IC_(50)value was 12.38 mg/mL.The results of this study indicate that nacre antioxidant peptides have a good application prospect in the field of food and cosmetics,and provide a theoretical basis for the high-value utilization of shell nacre.

关 键 词：珍珠层 活性肽 抗氧化活性 酪氨酸酶抑制活性 响应面分析 氨基酸组成 

分 类 号：TS201.1[轻工技术与工程—食品科学]