纳豆激酶生产菌株选育及其发酵培养基优化  

Breeding of Nattokinase-Producing Strains and Optimization of Fermentation Medium

作　　者：文雯 胡海峰 WEN Wen;HU Haifeng(Yangling Vocational&Technical College,Yangling,Shaanxi 712100,China;China Pharmaceutical University,Nanjing,Jiangsu 211100,China;Sinopharm Health Industry Institute Co.,Ltd.,Shanghai 201203,China)

机构地区：[1]杨凌职业技术学院,陕西杨凌712100 [2]中国药科大学,江苏南京211100 [3]国药集团健康产业研究院,上海201203

出　　处：《陕西农业科学》2025年第2期9-16,共8页Shaanxi Journal of Agricultural Sciences

基　　金：陕西省重点研发计划项目(2023-YBNY-249);陕西省教育厅青年创新团队建设项目(21JP143);杨凌职业技术学院2023年校内科研基金项目(DT2023-001)。

摘　　要：以实验室保藏的纳豆芽孢杆菌SIPI-W-N5为出发菌株,通过紫外诱变和DES诱变,获得了突变株SIPI-W-N5-16,采用响应面优化试验对其发酵培养基进行优化。结果表明,突变株SIPI-W-N5-16较出发菌株产纳豆激酶活力提高了149.24%,并确定了该突变株最佳摇瓶发酵配方,采用该发酵培养基发酵突变株SIPI-W-N5-16,其产纳豆激酶活力较优化前提高了104%。Using a Bacillus subtilis SIPI-W-N5 strain preserved under laboratory conditions as the starting strain,a mutant strain SIPI-W-N5-16 was obtained through ultraviolet(UV)and diethyl sulfate(DES)mutagenesis.Response surface methodology(RSM)experiments were conducted to optimize the fermentation medium for the mutant strain.The results showed that the nattokinase activity of SIPI-W-N5-16 was 149.24% higher than that of the original strain.The optimal fermentation medium for shake flask cultivation of the mutant strain was determined.Fermentation of SIPI-W-N5-16 with the optimized medium led to a 104% enhancement in nattokinase activity compared to pre-optimization conditions.

关 键 词：纳豆激酶 发酵 菌株选育 工艺优化 响应面试验 

分 类 号：TQ925[轻工技术与工程—发酵工程]