高产AI-2副溶血性弧菌的筛选及毒力特性研究  

Screening and Virulence Characterization of Vibrio parahaemolyticus with High Production of AI-2

作　　者：袁硕 王彦博 刘水琳 崔方超 檀茜倩 吕欣然 励建荣 李学鹏 董浩 YUAN Shuo;WANG Yanbo;LIU Shuilin;CUI Fangchao;TAN Xiqian;LÜ Xinran;LI Jianrong;LI Xuepeng;DONG Hao(College of Food Science and Engineering,Bohai University,Fresh Agricultural Products Storage and Processing and Safety Control Technology National and Local Joint Engineering Research Center,Liaoning Key Laboratory of Food Safety,Jinzhou 121013,Liaoning;Dalian Customs Technology Center,Dalian 116034,Liaoning;Dalian Polytechnic University Marine Food Deep Processing Key Technology Co-construction Collaborative Innovation Center,Dalian 116034,Liaoning;Shandong Meijia Group Co.,LTD.,Rizhao 276800,Shandong)

机构地区：[1]渤海大学食品科学与工程学院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁省食品安全重点实验室,辽宁锦州121013 [2]大连海关技术中心,辽宁大连116034 [3]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [4]山东美佳集团有限公司,山东日照276800

出　　处：《中国食品学报》2025年第2期115-123,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2019YFD0901702);辽宁省教育厅面上项目(JYTMS20231621)。

摘　　要：副溶血性弧菌是一种常见的食源性致病菌,其运动能力、生物被膜形成和毒力因子产生等受AI-2/LuxS型群体感应系统调控。本研究从9株副溶血性弧菌中筛选具有高产AI-2信号分子能力的菌株,通过添加外源AI-2信号分子分析其对生物被膜、毒力因子和运动性等的影响。结果表明,获得1株高产AI-2信号分子的副溶血性弧菌VP-4,添加不同体积分数的外源AI-2信号分子(1%~7%)对其生物被膜、明胶酶、卵磷脂酶、淀粉酶、运动能力和溶血性的促进率分别为33.02%~67.87%,1.67%~7.26%,3.25%~48.15%,1.21%~12.17%,4.92%~35.98%,4.15%~50.60%,2.06%~15.23%。同时,扫描电镜观察发现添加外源AI-2信号分子的副溶血性弧菌生物被膜菌体聚集堆积,厚度增加,结构致密。上述结果说明AI-2信号分子可以促进副溶血性弧菌生物被膜的形成及毒力因子的释放,提高其运动能力。本研究可为控制海产品中副溶血性弧菌的致病性提供新靶点。Vibrio parahaemolyticus is a common foodborne pathogen whose motility,biofilm formation and virulence factor production are regulated by AI-2/LuxS quorum sensing system.In this study,V.parahaemolyticus with high yield of AI-2 signaling molecules was selected from nine strains Vibrio,and effects of exogenous AI-2 signaling molecules on biofilm,virulence factors and motility of V.parahaemolyticus were analyzed.The results showed that a strain of V.parahaemolyticus VP-4 with high production of AI-2 signaling molecules was obtained,and the promotion rates of biofilm,gelatinase,lecithin enzyme,amylase,motility,and hemolysis by adding exogenous AI-2 signaling molecules with different volume fractions(1%-7%)were 33.02%-67.87%,1.67%-7.26%,3.25%-48.15%,1.21%-12.17%,4.92%-35.98%,4.15%-50.60%,and 2.06%-15.23%,respectively.Meanwhile,scanning electron microscope observation showed that the addition of exogenous AI-2 signaling molecules caused biofilm of V.parahaemolyticus to accumulate,increased in thickness and compact structure.The above results indicated that AI-2 signaling molecules could promote the formation of V.parahaemolyticus biofilm and the release of virulence factors,as well as enhance its motility.This study could provide a novel target for controlling the pathogenicity of Vibrio parahaemolyticus in seafood.

关 键 词：海产品 副溶血性弧菌 AI-2/LuxS 生物被膜 毒力因子 

分 类 号：TS201.3[轻工技术与工程—食品科学]