不同培养条件下蜡样芽胞杆菌AI-2和蛋白酶的相关性  

The Correlation between Bacillus cereus AI-2 and Protease at Different Culture Conditions

作　　者：祁雪鹤 贾嫒 黄燕 宋丹靓敏 秦雪 张微 姜毓君[1] 满朝新[1] 杨鑫焱 QI Xuehe;JIA Ai;HUANG Yan;SONG Danliangmin;QIN Xue;ZHANG Wei;JIANG Yujun;MAN Chaoxin;YANG Xinyan(College of Food Science and Technology,Northeast Agricultural University,Harbin 150030)

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《中国食品学报》2025年第2期134-142,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金区域创新发展联合基金项目(U21A20272)。

摘　　要：以分离自原料乳的1株产蛋白酶高酶活性的蜡样芽胞杆菌D8为研究对象,通过外源添加信号分子AI-2和改变菌株的培养条件(温度、pH值、渗透压、营养条件),明确AI-2和蛋白酶间的关系,并探究菌株AI-2产量和蛋白酶活性的相关性。采用高效液相色谱法和偶氮酪蛋白法检测上清液中信号分子AI-2浓度和蛋白酶活性。结果表明,在不同温度、pH值和渗透压下,信号分子AI-2的浓度与蛋白酶活性变化趋势相同,呈正相关关系。在低营养胁迫下可以诱导蜡样芽胞杆菌产生更多的信号分子AI-2,蜡样芽胞杆菌蛋白酶活性降低,二者呈负相关关系。结论:不同培养条件影响信号分子AI-2产量和蛋白酶活性,二者存在相关性。A strain of Bacillus cereus D8 isolated from raw milk with high enzyme activity in protease production was taken as the research object.The relationship between AI-2 and protease was clarified by adding signal molecule AI-2 and changing the culture conditions of the strain(temperature,pH,osmotic pressure,nutritional conditions),and the correlation between the production of AI-2 and protease activity of the strain was explored.The concentration of signal molecule AI-2 and the activity of protease in supernatant were detected by HPLC and azo casein method.The results showed that the concentration of AI-2 was positively correlated with the activity of protease at different temperature,pH and osmotic pressure.Under hypotrophic stress,Bacillus cereus could induce more signal molecule AI-2,and the protease activity of Bacillus cereus decreased,and the two were negatively correlated.Conclusion:Different culture conditions affect the production of signal molecule AI-2 and the activity of protease,and there is correlation between the two.

关 键 词：蜡样芽胞杆菌 培养条件 AI-2 蛋白酶活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]