Salacia fruticosa methanol extract pretreatment attenuates scopolamine-induced cognitive decline and Alzheimer's disease-related pathology in zebrafish  

作　　者：Praveen Kumar Pasala DSNBK Prasanth Siva Prasad Panda Vaishnavi Munnangi Sharon Blessy Sheikh F.Ahmad Haneen A.Al-Mazroua 

机构地区：[1]Department of Pharmacology,Raghavendra Institute of Pharmaceutical Education and Research,JNTUA,Anantapuramu,Andhra Pradesh 515721,India [2]School of Pharmacy&Technology Management,SVKM's Narsee Monjee Institute of Management Studies,Polepally SEZ,TSIIC,Jadcherla,Mahbubnagar,Hyderabad,Telangana 509301,India [3]Department of Pharmacology,Institute of Pharmaceutical Research,GLA University,Mathura,Uttar Pradesh,India [4]Department of Pharmacology and Toxicology,College of Pharmacy,King Saud University,Riyadh 11451,Saudi Arabia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2025年第3期109-118,I0002-I0015,共24页亚太热带生物医学杂志(英文版)

基　　金：funded by King Saud University,Riyadh,Saudi Arabia,Project Number(RSPD2025R709).

摘　　要：Objective:To examine the effect of the methanolic extract of Salacia fruticosa in a zebrafish model of scopolamine-induced Alzheimer’s disease.Methods:High-resolution liquid chromatography-mass spectrometry was used to characterize the phytochemical constituents of Salacia fruticosa methanolic extract.The drug-likeness of these compounds was determined via the DruLiTo tool,and their acetylcholinesterase(AChE)binding affinities were studied by molecular docking.In in vivo studies,adult zebrafish were treated with 3.125,6.25,and 12.5 mg/L of the extract for seven days and then immersed in scopolamine(100μM/L)to induce cognitive deficits.T-maze and novel object recognition tests were used for behavioral studies.In addition,the activities of AChE,antioxidant enzymes,and myeloperoxidase were determined in brain tissue of zebrafish.Results:High-resolution liquid chromatography-mass spectrometry revealed that 40 phytoconstituents were present in the methanolic extract of Salacia fruticosa,and 27 compounds met Lipinski's rule of five,indicating good drug-likeness.Some compounds such as stylopine,p-coumaroylagmatine,and(-)-heliannuol E,demonstrated high AChE binding affinity.Moreover,pretreatment with the extract significantly mitigated zebrafish cognitive decline,as indicated by increased time spent at the novel object in novel object recognition test,as well as increased time spent and decreased latency in the green arm(P<0.001).The extract also markedly lowered malondialdehyde and myeloperoxidase levels and AChE activity,and enhanced glutathione peroxidase and superoxide dismutase activities(P<0.001)in zebrafish with scopolamine-induced Alzheimer’s disease.Histopathological studies revealed that Salacia fruticosa extract ameliorated scopolamine-induced abnormalities in neuronal cell morphology.Conclusions:Pretreatment with the methanolic extract of Salacia fruticosa reduces cognitive impairment,enhances antioxidants,and attenuates oxidative stress,highlighting its potential as a preventive agent for Alzheimer’s

关 键 词：SCOPOLAMINE Acetylcholinesterase inhibitors Salacia fruticosa ZEBRAFISH Molecular docking ANTIOXIDATION NEUROINFLAMMATION 

分 类 号：R74[医药卫生—神经病学与精神病学]