Impact of SLC16A8 on tumor microenvironment and angiogenesis in colorectal cancer: New therapeutic target insights  

作　　者：Hong-Peng Tian Zhong-Xiang Xiao Bo-Wen Su Yi-Xuan Li Hong Peng Chang-Yuan Meng 

机构地区：[1]Second Department of Gastrointestinal Surgery,The Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China [2]Department of Premarital and Prenatal Examination,Nanchong Shunqing District Maternal and Child Health Hospital,Nanchong 637000,Sichuan Province,China [3]Department of Anorectal Surgery,Nanchong Central Hospital,The Second Clinical Medical College,North Sichuan Medical College,Nanchong 637000,Sichuan Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第4期300-317,共18页世界胃肠肿瘤学杂志(英文)

基　　金：Supported by Nanchong Science and Technology Plan Project,No.23JCYJPT0064;Project of Sichuan Provincial Administration of Traditional Chinese Medicine,No.2024MS590。

摘　　要：BACKGROUND SLC16A8,a lactate efflux transporter,is upregulated in various cancers,but its effects on tumor microenvironments remain understudied.This research explores its role in colorectal cancer(CRC)and the impact on the associated microenvir-onment consisting of vascular endothelial cells.AIM To explore the role in CRC and the impact on the associated microenvironment consisting of vascular endothelial cells.METHODS Hypoxic conditions prompted examination of SLC16A8 expression,glycolysis,lactate efflux,and Warburg effect correlations in CRC cell lines.Co-culture with HUVEC allowed for endothelial-mesenchymal transition(EndMT)character-ization,revealing lactate efflux's influence.Knockdown of SLC16A8 in CRC cells enabled relevant phenotype tests and tumorigenesis experiments,investigating tumor growth,blood vessel distribution,and signaling pathway alterations.RESULTS SLC16A8 expression was significantly upregulated in CRC tissues compared to adjacent normal tissues and correlated with disease progression(P<0.05).Under hypoxic conditions,HIF-1αinduced SLC16A8 expression,leading to enhanced metabolic reprogramming and increased lactate production.siRNA-mediated SLC16A8 knockdown effectively reversed hypoxia-induced changes,including reduced glucose consumption and lactate production.Co-culture experiments revealed that SLC16A8 knockdown significantly inhibited hypoxia-induced EndMT in HUVEC cells.In vivo studies demonstrated that SLC16A8 knockdown suppressed tumor growth,reduced Ki67 expression,and decreased HIF-1αlevels.Furthermore,SLC16A8 silencing led to decreased ex-pression of key metabolic enzymes PKM2 and LDHA,indicating its role in glycolytic regulation.CONCLUSION Our findings reveal that SLC16A8 functions as a critical mediator of hypoxia-induced metabolic reprogramming in CRC progression.

关 键 词：SLC16A8 Colorectal cancer HYPOXIA GLYCOLYSIS ANGIOGENESIS 

分 类 号：R73[医药卫生—肿瘤]