生物被膜增强食源性致病菌耐药性和毒力的研究进展  

Research Progress on the Role of Biofilms in Enhancing Antibiotic Resistance and Virulence in Foodborne Pathogens

作　　者：孙露 李云 王少飞 姜祖徉 张鸿林 吴博文 董庆利[1] 王翔[1] SUN Lu;LI Yun;WANG Shaofei;JIANG Zuyang;ZHANG Honglin;WU Bowen;DONG Qingli;WANG Xiang(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093

出　　处：《食品科学》2025年第8期363-371,共9页Food Science

基　　金：上海市科技兴农项目(2022-02-08-00-12-F01144)。

摘　　要：生物被膜是附着在生物或非生物表面的微生物群落,其形成显著提高了食源性致病菌的生存能力。生物被膜不仅通过物理屏障有效阻隔抗生素的渗透,还通过调节基因表达和促进物质交换等方式增强了致病菌对抗生素的耐受能力;生物被膜通过调控毒力基因的表达,常导致致病菌毒力的升高。此外,生物被膜中的群体感应系统和环二鸟苷酸信号系统在调控耐药性和毒力方面也发挥了关键作用,而非编码小RNAs在维持生物被膜稳定性和抗逆性中也扮演了重要角色。这些机制协同作用提升了食源性致病菌在不利环境中的生存能力。因此,深入探究食源性致病菌生物被膜对抗生素耐药性和毒力的影响及机制,对制定安全有效的防控策略具有重要意义。本综述围绕食源性致病菌形成生物被膜后其抗生素耐药性和毒力变化开展,并探讨生物被膜在基因和细胞水平上对耐药性和毒力的影响机制,以期为深入了解食源性致病菌生物被膜引起的风险提供参考,为食品加工工业中生物被膜危害防控工作提供理论支持,为开发新型抗菌策略和改进食品安全管理措施提供科学依据。Biofilms,which are microbial communities that adhere to biological or abiotic surfaces,significantly enhance the survival capacity of foodborne pathogens.Biofilms not only effectively block the penetration of antibiotics through physical barriers but also increase the tolerance of pathogens to antibiotics by regulating gene expression and promoting material exchange.Furthermore,biofilms often enhance the pathogenicity of pathogens by upregulating the expression of virulence genes.In addition,the quorum sensing system and cyclic dinucleotide signaling pathway in biofilms play key roles in regulating antibiotic resistance and virulence.Non-coding small RNAs are crucial for maintaining biofilm stability and stress resistance.These mechanisms work in synergy to improve the survival of foodborne pathogens under adverse environmental conditions.Therefore,understanding the influence and mechanism of biofilm formation on antibiotic resistance and virulence in foodborne pathogens is essential for developing safe and effective control strategies.This review focuses on the changes in antibiotic resistance and virulence in foodborne pathogens after biofilm formation and explores the molecular and cellular mechanisms through which biofilms affect resistance and virulence.It aims to provide insights into the risks associated with foodborne pathogen biofilms,offer theoretical support for biofilm-related hazard control in the food processing industry,and provide a scientific basis for developing new antimicrobial strategies and improving food safety management practices.

关 键 词：食源性致病菌 生物被膜 抗生素耐药 毒力 

分 类 号：TS201.3[轻工技术与工程—食品科学]