牡蛎酶解产物对糖皮质激素诱导骨质疏松大鼠的改善作用  

Ameliorative effect of oyster enzymatic products on glucocorticoidinduced osteoporosis in rats

作　　者：马文语 杨维[1] 秦小明[1,2] 曹文红 林海生[1,2] MA Wenyu;YANG Wei;QIN Xiaoming;CAO Wenhong;LIN Haisheng(School of Food Science and Technology,Guangdong Ocean University/National R&D Center for Shellfish Processing Technology(Zhanjiang)/Key Research Laboratory of Aquatic Products Processing and Safety of Guangdong Province/Marine Bioproducts Engineering Laboratory of Guangdong Province/Guangdong Food Engineering and Technology Research Center/Guangdong Provincial Aquatic Prepared Processing and Quality Control Engineering Technology Center,Zhanjiang 524088,China;Shenzhen Research Institute,Guangdong Ocean University,Shenzhen 518120,China)

机构地区：[1]广东海洋大学食品科技学院/国家贝类加工技术研发中心(湛江)/广东省水产品加工与安全重点研究室/广东省海洋生物制品工程实验室/广东省食品工程技术研究中心/广东省水产预制加工与品质控制工程技术中心,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518120

出　　处：《南方水产科学》2025年第2期182-190,共9页South China Fisheries Science

基　　金：国家现代农业产业技术体系(CARS-49)。

摘　　要：牡蛎具有多种生物活性,随着对其活性开发的深入研究,发现其在骨质疏松的改善方面有巨大潜力。以牡蛎酶解产物(Oyster enzymatic products,OP)为研究对象,探讨其对糖皮质激素性骨质疏松症(Glucocorticoids osteoporosis,GIOP)的缓解作用。通过建立地塞米松诱导的GIOP模型,使用不同剂量的OP进行干预,从血清生化指标、微型CT(Micro-CT)、三维重建及组织病理切片等方面评估其对大鼠骨质疏松症的缓解作用。结果发现,与模型组对比,OP低、中、高剂量组的血清中成骨细胞标志物骨保护素、I型前胶原氨基端肽和睾酮平均提高了13.67%、11.51%和17.80%(p<0.05);破骨细胞标志物基质金属蛋白酶、组织蛋白酶K和抗酒石酸酸性磷酸酶活性平均下降了15.44%、31.20%和14.43%(p<0.05);骨密度测定、Micro-CT和股骨组织病理分析结果显示,OP高剂量组的骨密度、骨小梁面积占比和骨盐沉积面积分别比模型组增加了56.31%、18.59%和22.51%(p<0.05)。综上,OP可通过提高成骨细胞合成骨胶原能力来加速新骨的形成,降低骨基质降解速度和骨代谢的速度,减少骨量流失,并提高性激素水平从而增加大鼠的骨密度、骨体积分数,增强骨的钙盐沉积能力,改善地塞米松诱导的糖皮质激素性骨质疏松症状。Oyster has a variety of biological activities,and with the intensive research on the development of its activities,the great potential for the improvement of osteoporosis has been found.In this paper,the oyster enzymatic products(OP)were used to investigate their mitigating effects on glucocorticoid-type osteoporosis.A dexamethasone-induced glucocorticoids osteopo-rosis(GIOP)model was established,and different doses of OP were used to evaluate the mitigating effects of OP on the osteopo-rosis in rats in terms of serum biochemical indexes,Micro-CT,three-dimensional reconstruction,and histopathological sections.The results show that compared with the model group,the serum content of osteoprotegerin,Type I procollagen amino-termin-al peptide and testosterone,which are the osteoclast markers,increased by an average of 13.67%,11.51%and 17.80%in the low,medium and high dose groups,respectively(p<0.05);but the serum content of matrix metalloproteinases,histone K and anti-tartrate acid phosphatase,which are the osteoclast markers,decreased by 15.44%,31.20%and 14.43%,respectively(p<0.05).The results of bone densitometry and histopathological analysis of Micro-CT and femur indicate that compared with those of the model group,the bone mineral density,the percentage of bone trabecular area and the area of bone salt deposition in the high-dose group increased by 56.31%,18.59%and 22.51%,respectively(p<0.05).In conclusion,OP can accelerate the forma-tion of new bone by increasing the ability of osteoblasts to synthesize collagen,decrease the rate of bone matrix degradation and bone metabolism,decrease the loss of bone mass,increase the level of sex hormones,increase the BMD,bone volume fraction,enhance the ability of bone to deposit bone calcium salts in rats,thus improve the symptoms of glucocorticoid-induced osteo-porosis induced by dexamethasone.

关 键 词：牡蛎 酶解产物 骨质疏松症 糖皮质激素 骨密度 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]