茶枝柑果皮类黄酮抑制AGEs的效果及作用机制  

Inhibitory Effects of Flavonoids from Citrus reticulata'Chachi'Peel on the Formation of Advanced Glycation End-products

作　　者：王婉婷 陈婉冰 朱从一 杨梦雪 朱国辉[2] 曾继吾[1] WANG Wanting;CHEN Wanbing;ZHU Congyi;YANG Mengxue;ZHU Guohui;ZENG Jiwu(Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640,China;College of Life Sciences,South China Agricultural University,Guangzhou 510630,China)

机构地区：[1]广东省农业科学院果树研究所,农业农村部南亚热带果树生物学与遗传资源利用重点实验室,广东省果树科学与技术研究重点实验室,广东广州510640 [2]华南农业大学生命科学学院,广东广州510630

出　　处：《食品工业科技》2025年第9期153-164,共12页Science and Technology of Food Industry

基　　金：国家现代农业(柑橘)产业技术体系(CARS-26);广东省乡村振兴战略专项(2022-NPY-00-033,2022-NBH-00-017)。

摘　　要：本研究采用牛血清白蛋白-葡萄糖(bovine serum albumin-glucose,BSA-Glu)模拟体系,研究不同生长期的茶枝柑果皮提取物及主要类黄酮化合物川陈皮素、橘皮素和橙皮苷对荧光性AGEs的抑制效果,同时,借助多光谱技术和计算机分子对接研究类黄酮与BSA的相互作用,探究其抑制AGEs的作用机制。结果表明,4个不同生长期的茶枝柑果皮提取物均对AGEs的生成具有显著抑制作用,12月茶枝柑果皮提取物(2.5 mg/mL)对荧光性AGEs以及蛋白糖氧化产物二酪氨酸、犬尿氨酸和N'-甲酰基犬尿氨酸的最大抑制率分别为76.33%、71.78%、62.73%和66.07%。荧光光谱表明,川陈皮素、橘皮素和橙皮苷能静态猝灭BSA的内源性荧光。同步荧光光谱表明,川陈皮素、橘皮素和橙皮苷能轻微改变BSA的构象以及色氨酸(TRP)残基和酪氨酸(TYR)残基的微环境。分子对接表明,川陈皮素、橘皮素和橙皮苷可结合到BSA的TRP-213残基附近的疏水性口袋并与BSA形成复合物,同时与BSA的多个氨基酸残基之间存在氢键和疏水相互作用,阻碍BSA糖基化位点与葡萄糖结合。由此可见,茶枝柑果皮类黄酮通过减少精氨酸、赖氨酸残基与还原糖的结合的方式抑制AGEs的生成,可用于AGEs天然抑制剂的开发。This study investigated the inhibitory effects of extracts from Citrus reticulata'Chachi'peels at different growth stages and the primary flavonoid components(nobiletin,hesperetin,and hesperidin)on fluorescent AGEs using the bovine serum albumin-glucose(BSA-Glu)model.To elucidate the underlying mechanisms,the interactions between flavonoids and BSA were analyzed using multi-spectral techniques and computer molecular docking.Results showed that extracts from Citrus reticulata'Chachi'peels at four growth stages significantly inhibited AGEs formation.The extracts obtained in December(2.5 mg/mL)showed maximum inhibition rates of 76.33%for fluorescent AGEs and 71.78%,62.73%,and 66.07%for the protein glycoxidation products dityrosine,kynurenine,and N'-formylkynurenine,respectively.Fluorescence spectroscopy revealed that the fluorescence quenching mechanism of BSA by nobiletin,hesperetin,and hesperidin was via a static quenching procedure.Synchronous fluorescence spectroscopy revealed that these flavonoids slightly altered the conformation of BSA and the microenvironment of tryptophan(TRP)and tyrosine(TYR)residues.Molecular docking studies showed that the flavonoids bound to a hydrophobic pocket near the TRP-213 residue of BSA,forming a complex and disrupting glucose binding to glycation sites on BSA through hydrogen bonds and hydrophobic interactions.In conclusion,flavonoids derived from Citrus reticulata'Chachi'peels effectively inhibited the formation of AGEs by reducing the combination of arginine and lysine residues with reducing sugars,and had potential for use in developing natural AGEs inhibitors.

关 键 词：茶枝柑 类黄酮 相互作用 晚期糖基化终产物 抑制 分子对接 

分 类 号：TS201.2[轻工技术与工程—食品科学]