复合酶解法提取石榴皮不溶性膳食纤维工艺条件优化  

Optimization of process conditions for extracting insoluble dietary fiber from pomegranate peel by compound enzymatic hydrolysis

作　　者：马玉坤 邓俊熙 杨得梅 吴妍 刘太林[1] MA Yukun;DENG Junxi;YANG Demei;WU Yan;LIU Tailin(School of Food Engineering,Tianjin Tianshi College,Tianjin 301700,China)

机构地区：[1]天津天狮学院食品工程学院,天津301700

出　　处：《安徽农学通报》2025年第8期90-94,共5页Anhui Agricultural Science Bulletin

基　　金：国家级大学生创新创业训练计划项目(202310859007)。

摘　　要：本试验以石榴皮为原料,采用酶解法从果皮的膳食纤维中分离出不溶性膳食纤维(IDF),以单因素和正交试验方法探究果胶酶添加量、木瓜蛋白酶添加量、纤维素酶添加量、酶解温度和水浴时间对IDF得率的影响,并进行工艺条件优化。单因素试验结果表明,果胶酶、木瓜蛋白酶和纤维素酶的最佳添加量分别为1.00%、1.25%和0.75%,最佳酶解温度50℃,最佳酶解时间60min。正交优化试验确定酶法提取石榴皮IDF的最佳工艺条件:在料液比1∶25(m∶V),纤维素酶添加量0.75%的条件下,果胶酶和木瓜蛋白酶添加量均为1.00%最佳复合酶配比为1∶1,酶解温度50℃,水浴时间60min;在此条件下,IDF得率达47.93%,持水力、溶胀性分别为3.43g/g、3.45mL/g。本研究为后续获取膳食纤维并合理利用提供参考。Pomegranate peel was used as the raw material and separated insoluble dietary fiber(IDF)from the dietary fiber of the peel using enzymatic hydrolysis.Single factor and orthogonal experimental methods were used to investigate the effects of pectinase addition,papain addition,cellulase addition,enzymatic hydrolysis temperature,and water bath time on the yield of IDF,and to optimize the process conditions.The results of the single factor experiment showed that the optimal addition amounts of pectinase,papain,and cellulase were 1.00%,1.25%,and 0.75%,respectively.The optimal enzymatic hydrolysis temperature was 50℃,and the optimal enzymatic hydrolysis time was 60 min.Orthogonal optimization experiment was conducted to determine the optimal process conditions for enzymatic extraction of pomegranate peel IDF:under the condition of a solid-liquid ratio of 1∶25(m∶V)and addition amount of cellulase 0.75%,the addition of pectinase and papain were both 1.00%.The optimal composite enzyme ratio was 1∶1,the enzymatic hydrolysis temperature was 50℃,and the water bath time was 60 min;Under these conditions,the yield of IDF reached 47.93%,with hydraulic holding capacity and swelling capacity of 3.43 g/g and 3.45 mL/g,respectively.This study provides a reference for the rational utilization of dietary fiber in the future.

关 键 词：石榴皮 水不溶性膳食纤维 正交法 复合酶解法 

分 类 号：TS209[轻工技术与工程—食品科学]