信阳商城筒鲜鱼中乳酸菌的分离及鉴定  

Isolation and identification of lactic acid bacteria from Tongxian fish in Shangcheng county of Xinyang

作　　者：陈亚蓝 陈顺心 邢淑婕 朱静[1,2] 余彪 CHEN Yalan;CHEN Shunxin;XING Shujie;ZHU Jing;YU Biao(College of Food Science and Engineering,Xingyang Agriculture and Forestry University,Xinyang 464000,China;Dabie Mountain Characteristic Food Resources Comprehensive,Utilization Engineering Technology Research Center,Xinyang 464000,China;Luohe Vocational Technology College,Luohe 462000,China)

机构地区：[1]信阳农林学院食品科学与工程学院,河南信阳464000 [2]河南省大别山特色食物资源综合利用工程技术研究中心,河南信阳464000 [3]漯河职业技术学院,河南漯河462000

出　　处：《中国酿造》2025年第4期159-164,共6页China Brewing

基　　金：河南省重点研发专项(231111312800);河南省高校研究性教学改革与实践研究项目(2022SYJXLX119);河南省高等学校重点科研项目计划(24B550017,24B180016);河南省2024年科技发展计划(242102110090);信阳农林学院科技创新团队(XNKJTD-001);信阳农林学院青年基金项目(2019LG009)。

摘　　要：该研究通过稀释涂布平板法、划线分离法从发酵5 d、10 d和15 d的信阳商城筒鲜鱼中分离纯化乳酸菌,并通过形态学观察、生理生化试验筛选发酵特性优良的乳酸菌。通过分子生物学技术对筛选菌株进行鉴定,并对其生长及产酸性能进行分析。结果表明,从筒鲜鱼中共分离纯化出19株菌株,其中12株为乳酸菌。12株乳酸菌均不产粘,发酵葡萄糖、麦芽糖、乳糖和蔗糖均产酸不产气,氨基酸脱羧酶试验结果均为阴性,均能水解淀粉,其中,菌株L15-9的水解淀粉能力、耐NaCl、亚硝酸盐能力及抑菌能力最强。经鉴定,菌株L15-9为一株植物乳植杆菌(Lactiplantibacillus plantarum),其能在培养2 h后迅速进入对数生长期,12 h后进入稳定生长期,并在12 h时pH值降低至4.0以下。In this study,lactic acid bacteria were isolated and purified from Tongxian fish in Shangcheng county of Xinyang fermented for 5 d,10 d and 15 d by dilution plate method and streak separation method,and lactic acid bacteria with excellent fermentation characteristics were screened by morphological observation and physiological and biochemical tests.The screened strains were identified by molecular biology techniques,and their growth and acid-producing properties were analyzed.The results showed that 19 strains were isolated and purified from fresh fish,12 of them were lactic acid bacteria,all of which produced no viscosity,fermented glucose,maltose,lactose and sucrose,produced acid without gas,amino acid decar-boxylase test results were negative,and could hydrolyze starch,among which strain L15-9 had the strongest starch hydrolysis ability,NaCl tolerance,nitrite tolerance and bacteriostasis ability.The results showed that a total of 19 strains were isolated and purified from Tongxian fish,of which 12 were lactic acid bacteria.All 12 strains did not produce viscosity,fermented glucose,maltose,lactose and sucrose produced acid but no gas.The re-sults of amino acid decarboxylase test were negative,and all strains could hydrolyze starch.Among them,strain L15-9 had the strongest capacity of starch hydrolysis,NaCl resistance,nitrite resistance and antibacterial ability.Strain L15-9 was identified as Lactiplantibacillus plantarum.It rapidly entered a logarithmic growth phase after 2 h and a stable growth phase after 12 h,and its pH decreased to below 4.0 at 12 h.

关 键 词：筒鲜鱼 乳酸菌 分离 筛选 鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]