TTC应用于食品菌落总数检测的研究  

Research on the Application of TTC in the Detection of Total Colony Count in Food

作　　者：黄幼娜 HUANG Youna(Luoding Center for Disease Control and Prevention,Luoding 527200,China)

机构地区：[1]罗定市疾病预防控制中心,广东罗定527200

出　　处：《食品安全导刊》2025年第11期57-59,共3页China Food Safety Magazine

基　　金：云浮市医药卫生科研立项项目(2023B059)。

摘　　要：为探究食品菌落总数检测中的最佳氯化三苯甲基四氮唑(2,3,5-Triphenyltetrazolium Chloride,TTC)溶液浓度范围,本文研究了不同食品、不同菌种与添加不同TTC浓度溶液的平板计数琼脂中的菌落生长情况。结果显示,食品菌落总数检测中最佳的TTC添加溶液浓度范围为0.001%~0.002%,既能使菌落显色便于计数,也不抑制菌落的生长。To obtain the optimal concentration range of 2,3,5-triphenyltetrazolium chloride(TTC)solution for total bacterial count detection in food,the study investigates the colony growth of different bacterial strains grown in various foods on agar plates with diverse TTC concentration.The result show that the optimal concentration range of TTC addition solution in the total colony count detection of food is 0.001% to 0.002%,which can not only make the colonies colored for easy counting but also does not inhibit the growth of colonies.

关 键 词：食品 菌落总数 氯化三苯甲基四氮唑(TTC) 

分 类 号：TS207.4[轻工技术与工程—食品科学]