Optogenetic approaches for neural tissue regeneration:A review of basic optogenetic principles and target cells for therapy  

作　　者：Davletshin Eldar Sufianov Albert Ageeva Tatyana Sufianova Galina Rizvanov Albert Mukhamedshina Yana 

机构地区：[1]OpenLab Gene and Cell Technologies,Institute of Fundamental Medicine and Biology,Kazan Federal University,Kazan,Russia [2]Department of Neurosurgery,Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation(Sechenov University),Moscow,Russia [3]Research and Educational Institute of Neurosurgery,Peoples’Friendship University of Russia(RUDN),Moscow,Russia [4]Department of Pharmacology,Tyumen State Medical University,Tyumen,Russia [5]Division of Medical and Biological Sciences,Tatarstan Academy of Sciences [6]Department of Histology,Cytology and Embryology,Kazan State Medical University,Kazan,Russia

出　　处：《Neural Regeneration Research》2026年第2期521-533,共13页中国神经再生研究(英文版)

基　　金：supported by a grant from the Russian Science Foundation,No.23-75-10041(to MY)。

摘　　要：Optogenetics has revolutionized the field of neuroscience by enabling precise control of neural activity through light-sensitive proteins known as opsins.This review article discusses the fundamental principles of optogenetics,including the activation of both excitatory and inhibitory opsins,as well as the development of optogenetic models that utilize recombinant viral vectors.A considerable portion of the article addresses the limitations of optogenetic tools and explores strategies to overcome these challenges.These strategies include the use of adeno-associated viruses,cell-specific promoters,modified opsins,and methodologies such as bioluminescent optogenetics.The application of viral recombinant vectors,particularly adeno-associated viruses,is emerging as a promising avenue for clinical use in delivering opsins to target cells.This trend indicates the potential for creating tools that offer greater flexibility and accuracy in opsin delivery.The adaptations of these viral vectors provide advantages in optogenetic studies by allowing for the restricted expression of opsins through cell-specific promoters and various viral serotypes.The article also examines different cellular targets for optogenetics,including neurons,astrocytes,microglia,and Schwann cells.Utilizing specific promoters for opsin expression in these cells is essential for achieving precise and efficient stimulation.Research has demonstrated that optogenetic stimulation of both neurons and glial cells-particularly the distinct phenotypes of microglia,astrocytes,and Schwann cells-can have therapeutic effects in neurological diseases.Glial cells are increasingly recognized as important targets for the treatment of these disorders.Furthermore,the article emphasizes the emerging field of bioluminescent optogenetics,which combines optogenetic principles with bioluminescent proteins to visualize and manipulate neural activity in real time.By integrating molecular genetics techniques with bioluminescence,researchers have developed methods to monitor ne

关 键 词：adeno-associated virus ASTROCYTES bioluminescent optogenetics channelrhodopsins halorhodopsins MICROGLIA neural stem cells NEURONS OLIGODENDROCYTE OPTOGENETICS 

分 类 号：R741[医药卫生—神经病学与精神病学]