Sox2-overexpressing neural stem cells alleviate ventricular enlargement and neurological dysfunction in posthemorrhagic hydrocephalus  

作　　者：Baocheng Gao Haoxiang Wang Shuang Hu Kunhong Zhong Xiaoyin Liu Ziang Deng Yuanyou Li Aiping Tong Liangxue Zhou 

机构地区：[1]Department of Neurosurgery,West China Medical School,West China Hospital,Sichuan University,Chengdu,Sichuan Province,China [2]Department of Neurosurgery,The First People’s Hospital of Yunnan Province,The Affiliated Hospital of Kunming University of Science and Technology,Kunming,Yunnan Province,China [3]Department of Otolaryngology&Head and Neck Surgery,The First People’s Hospital of Yunnan Province,The Affiliated Hospital of Kunming University of Science and Technology,Kunming,Yunnan Province,China [4]State Key Laboratory of Biotherapy and Cancer Center,Research Unit of Gene and Immunotherapy,Chinese Academy of Medical Sciences,Collaborative Innovation Center of Biotherapy,West China Hospital,Chengdu,Sichuan Province,China [5]Department of Neurosurgery,NHC Key Laboratory of Nuclear Technology Medical Transformation(Mianyang Central Hospital),School of Medicine,University of Electronic Science and Technology of China,Mianyang,Sichuan Province,China [6]Department of Neurosurgery,Fifth People’s Hospital of Ningxia Hui Autonomous Region,Shizuishan,Ningxia Hui Autonomous Region,China

出　　处：《Neural Regeneration Research》2026年第2期769-779,共11页中国神经再生研究(英文版)

基　　金：supported by the National Natural Science Foundation of China,Nos.82473334(to LZ),82401629(to XL);the Major Scientific and Technological Achievements Transformation Project of Ningxia Hui Autonomous Region,No.2022CJE09013(to LZ);Mianyang Science and Technology Bureau(Mianyang Science and Technology Program),No.2023ZYDF097(to LZ);NHC Key Laboratory of Nuclear Technology Medical Transformation(Mianyang Central Hospital),No.2023HYX001(to LZ);Spinal Cord Diseases Clinical Medical Center of Yunnan Province,No.2024JSKFKT-16(to BG);the Natural Science Foundation of Sichuan Province,No.2024NSFSC1646(to XL);the China Postdoctoral Science Foundation,Nos.GZC20231811(to XL),2024T170601(to XL)and 2024M76228(to XL).

摘　　要：Neural stem cells(NSCs)have the potential for self-renewal and multidirectional differentiation,and their transplantation has achieved good efficacy in a variety of diseases.However,only 1%-10%of transplanted NSCs survive in the ischemic and hypoxic microenvironment of posthemorrhagic hydrocephalus.^(Sox2)is an important factor for NSCs to maintain proliferation.Therefore,^(Sox2)-overexpressing NSCs(NSC^(Sox2))may be more successful in improving neurological dysfunction after posthemorrhagic hydrocephalus.In this study,human NSC^(Sox2)was transplanted into a posthemorrhagic hydrocephalus mouse model,and retinoic acid was administered to further promote NSC differentiation.The results showed that NSC^(Sox2)attenuated the ventricular enlargement caused by posthemorrhagic hydrocephalus and improved neurological function.NSC^(Sox2)also promoted nerve regeneration,inhibited neuroinflammation and promoted M2 polarization(anti-inflammatory phenotype),thereby reducing cerebrospinal fluid secretion in choroid plexus.These findings suggest that NSC^(Sox2)rescued ventricular enlargement and neurological dysfunction induced by posthemorrhagic hydrocephalus through neural regeneration and modulation of inflammation.

关 键 词：ANGIOGENESIS cerebrospinal fluid hippocampal transplantation inflammation MICROGLIA neural stem cells NEUROGENESIS posthemorrhagic hydrocephalus retinoic acid ^(Sox2) 

分 类 号：R743.34[医药卫生—神经病学与精神病学]