肉及蛋类食品中氨基甲酸酯类农药多组分残留高效液相色谱分析  

Study on the tetermination of N-methylcarbamate insecticides in egg and meat by HPLC

作　　者：李萍[1] 王绪卿[1] 

机构地区：[1]中国预防医学科学院营养与食品卫生研究所,北京100050

出　　处：《卫生研究》2002年第6期465-467,共3页Journal of Hygiene Research

摘　　要：建立了动物性食品肉和蛋中氨基甲酸酯类农药 (NMCs)多组分残留高效液相色谱紫外检测 (HPLC-UV)分析方法。采用C1 8柱 ,甲醇 水 (6 0 :40 )为流动相 ,于 2 10nm进行检测。样品以丙酮 氯化钠 二氯甲烷一步法提取和分配 ,提取液浓缩后 (浓缩步骤须控制温度不超过 40℃ ) ,经过两次交联聚苯乙烯凝胶 (Bio BeadsS X3 )净化 ,收集流分为 5 0～ 80ml。 5种NMCs检测限为 3 2～ 13 3μg kg ,标准曲线线性良好 ,(r=0 991～ 0 997)。 5种NMCs标准平均过柱回收率为 89 8%～ 10 9 7% ,本方法净化效果良好。蛋和肉样中添加两种不同浓度的NMCs ,平均回收率分别为 81 6 %～ 97 2 %和 70 7%～ 94 0 %。A high-performance liquid chromatographic(HPLC) method using a reverse phase column(C 18)and methanol-water(60:40)mobile phase with ultraviolet detector (210nm) was established for simultaneous determination of 5 carbamate insecticides (aldicarb,MTMC,carbofuran,carbaryl and isoprocarb).The extraction and partition were combined into one step by saturating the extract with NaCl and driving the water away by dichloromethane.It was cleaned up twice by gel permeation(Bio-Beads S-X 3).The linearity for NMCs standards was good(r=0.991-0.997)and the detection limits of 5 carbamate insecticides were 3.2-13.3μg/kg.The recovery fortified with two levels of standard was in the range of 81.6% to 97.2% and 70.7% to 94.0% respectively.

关 键 词：蛋类食品 氨基甲酸酯 农药残留 高效液相色谱 肉类食品 

分 类 号：R155.51[医药卫生—营养与食品卫生学]