大米中桔青霉素的薄层色谱测定  

Determination of citrinin in rice by thin layer chromatography

作　　者：江涛[1] 张靖[2] 刘红蕾[2] 计融[2] 罗雪云[2] 

机构地区：[1]中国协和医科大学协和公共卫生学院,北京100021 [2]中国疾病预防控制中心营养与食品安全所

出　　处：《卫生研究》2002年第6期470-472,共3页Journal of Hygiene Research

摘　　要：建立了大米中桔青霉素的薄层色谱测定方法。大米样品用含 10 %乙醇酸的乙腈溶液提取 ,经过净化 ,再用三氯甲烷溶液定容至 1ml。薄层板浸泡在含草酸的甲醇溶液中进行预处理。大米样品中桔青霉素的加入水平为 10 0～ 10 0 0 μg kg时 ,回收率为 80 %～ 12 0 % ,方法的灵敏度为 30 μg kg ,最低检出量为 0 0 0A method is described for determining citrinin in rice.The rice sample is extracted with 10% glycolic acid and acetonitrile solution (1+9).The resulting solution is partially purified by several partition steps. Citrinin is determined by using thin layer chromatography.The chromatography plates are impregnated with oxalic acid by dipping them in an oxalic acid\|methanol solution before spot and development. Recovery of spiking citrinin from rice is 80%-120% at levels of 100-1000μg/kg.

关 键 词：大米 桔青霉素 薄层色谱 食品卫生 

分 类 号：R155.52[医药卫生—营养与食品卫生学]