离子排斥色谱法测定12种有机酸的规律  

Detection of Twelve Kinds of Organic Acids by Ion Exclusion Chromatography

作　　者：田鹏[1] 徐烨[2] 宋溪明[3] 梁志德[1] 

机构地区：[1]东北大学材料与冶金学院,辽宁沈阳110004 [2]东北大学理学院,辽宁沈阳110004 [3]辽宁大学化学系,辽宁沈阳110036

出　　处：《东北大学学报（自然科学版）》2003年第2期198-200,共3页Journal of Northeastern University(Natural Science)

基　　金：国家自然科学基金资助项目 ( 2 0 0 410 0 5 )

摘　　要：研究离子排斥色谱法分离测定有机酸 ,选用最常见的盐酸作淋洗液 ,以四丁基氢氧化铵为再生液 ,考察了淋洗液浓度、流量等因素对分离和测定的影响 ,对 12种有机酸在阴离子排斥色谱柱上的保留行为进行了系统的研究·指出HPICE AS1柱分离有机酸以离子排斥效应为主 ,同时也存在离子交换效应·通过实验确定最佳色谱条件 ,并在此条件下 ,测定啤酒中的有机酸·The separation and detection of popular organic acids by ion exclusion chromatography were developed. The effect of concentration and flow quantity of the eluant on the separation and detection was investigated by using common HCl acid as eluant and the TBA-OH as regenerant. The reservation of 12 kinds of organic acids in anion exclusion column was systematically stuied. It is shown that ion exclusion effect plays an important role on the separation identified by HPICE-AS1 column, while the ion exchange mechanism also plays a role. The optimum operation condition of the seperation and detection was established. On this condition, organic acids in a beer were tested successfully.

关 键 词：离子排斥色谱 有机酸 啤酒 柠檬酸 乳酸 乙酸 测定 

分 类 号：TS262.5[轻工技术与工程—发酵工程] TS207.3[轻工技术与工程—食品科学与工程]