酒精糟香菇深层发酵水溶性胞外香菇多糖的研究Ⅰ:分离纯化  

Studies On the water soluble polysaccharides Produced by Submerged Fermentation of Lentinus Edodes I : Seperation and Purification

作　　者：活泼 徐柔[2] 章克昌[2] 

机构地区：[1]浙江科技学院,浙江杭州310012 [2]江南大学生物工程学院,江苏无锡214036

出　　处：《酿酒》2003年第3期27-30,共4页Liquor Making

摘　　要：采用Sevag法和蛋白酶法结合除去蛋白质,以DEAE-纤维素对香茹多糖进行组别分离,分别以蒸馏水、0.01mol·L^(-1)、0.1mol·L^(-1)和0.2mol·L^(-1)硼砂溶液为洗脱剂进行洗脱得到四个组分,其中主要是组分Ⅰ,占总糖的76.9％。以Sepharose CL-6B凝胶色谱对组分I进行分级分离,在相对粘度1.5、样品上柱体积6mL、洗脱剂流速30mL·h^(-1)的条件下,组分Ⅰ被完全分离为两个组分XGA和XGB,XGA:XGB=1:9,XGB的高效法相色谱图显示为一单一对称峰。说明采用DEAE-纤维素和Sepharose CL-6B凝胶色谱分离纯化香菇多糖是可行的。The polysaccharides from the fermentation broth of Lentinus edodes were deproteined by Sevag and proteinase methods.And applied on DEAE- cellulose column,and stepwisely eluted with distilled water,0.01mol.L-1、0.1mol.L-1and0.2mol.L-1 borax solutions.Four fractions were obtained.Fraction I was the main fraction representing 76.9% of total sample amount.And Fraction I was subjected to chromatograph on Sepharose CL- 6B column, eluted with water at a flow rate of 30ml.h-1, two fractions XCA and XGB were obtained having a ratio of 1 ;9,HPLC analysis of XCB showed a mono - peak picture which indicated that polysaccharides could be separated and purified by DEAE - cellulose and Sepharose CL - 6B.

关 键 词：香菇多糖 酒精糟 深层发酵 Sevag法 蛋白酶法 分离纯化 DEAE-纤维素 凝胶色谱法 

分 类 号：TQ28[化学工程—有机化工]