大孔吸附树脂对酪蛋白非磷肽的脱盐和色谱分离  

The Application of Macroporous Adsorption Resin in Desalt and Chromatographic Separation of Casein Non-phosphate Peptides

作　　者：赵利[1] 王璋[1] 许时婴[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2003年第4期69-71,79,共4页Journal of Wuxi University of Light Industry

摘　　要：利用DA201 C大孔吸附树脂对酪蛋白非磷肽脱盐并进行分离分级.结果表明,大孔吸附树脂对酪蛋白非磷肽的脱盐率在97%以上,酪蛋白非磷肽的回收率约在95%,同时用不同体积分数的乙醇对酪蛋白非磷肽进行阶段洗脱,酪蛋白非磷肽均可以分离出3个组分.DA201C macroporous adsorption resin was used to desalt and casein nonphosphate peptides were separated with Sephadex G25. The result showed that the desalting ratio reached about 97%, whereas the recovery of casein nonphosphate peptides was about 95%. Three concentrations of different alcohol 30%, 50% and 70% were used to separate casein nonphosphate, and the peptides were fractionated to three peaks.

关 键 词：大孔吸附树脂 酪蛋白非磷肽 脱盐 分离 分级 酪蛋白 制备 

分 类 号：O657.7[理学—分析化学]