HPLC法柱后衍生测定乳制品中牛磺酸的含量  

Determination of taurine in dairy by HPLC derivation after column

作　　者：姜金斗[1] 刘波[1] 郝岩平[1] 

机构地区：[1]国家乳制品质量监督检验中心,黑龙江哈尔滨150086

出　　处：《中国乳品工业》2003年第4期47-49,共3页China Dairy Industry

摘　　要：建立了高效液相色谱法测定乳制品中牛磺酸含量的方法。采用荧光检测器,柱后OPA衍生剂衍生,色谱柱为Waerts氨基酸分析柱,柱温为25℃,流动相流速为0.4 mL/min,衍生剂流速为0.4 mL/min。标准曲线相关系数为0.9749;回收率为95.6％;8次样品测定SD为0.383 2,变异系数为1.2136％。A method for determination of taurine in dairy by HPLC is reported. Fluorscence detector, Waters-amino acid analysis column and derivation after column was used with column ternperature 25℃ and speed of mobile phase 0. 4 mL/ min and agent of derivation 0. 4 mL/min. The relative coefficient was 0. 9749, the recovery 95. 6% ; the SD in 8 sample by testing was 0. 3832 and CV= 1.2136%.

关 键 词：高效液相色谱法 测定 乳制品 牛磺酸 色谱仪 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]