DCA_(13)发酵产酸期代谢动力学模型的建立  

A Metabolic Kinetic Model for DCA_(13) Fermentation in DCA-producing Stage

作　　者：黄英明[1] 李春[1] 焦鹏[1] 曹竹安[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084

出　　处：《中国生物工程杂志》2003年第8期57-60,共4页China Biotechnology

基　　金：国家自然科学基金重点项目 ( 2 0 0 3 60 10 );国家自然科学基金资助项目 ( 3 0 0 0 0 0 0 3 )

摘　　要：根据产酸期烷烃代谢分析和底物质量平衡 ,针对使用Candidatropicalis生产十三碳二元酸反应体系 ,建立了热带假丝酵母在产酸期的代谢动力学模型 ,并对模型进行了数据拟合和实验验证。采用该代谢动力学模型 ,首先估算出在以烷烃为单一碳源的培养条件下 ,烷烃对二元酸的最高理论摩尔转化率为 64% ,最高理论质量转化率为 85 % ,在此基础上 ,进一步提出补加其它碳源是提高烷烃转化率最为有效的措施。A metabolic kinetic model for DCA production in the DCA-producing stage was constructed on the basis of the substrate balance and metabolic flux analyses. The results of data simulation and experiments confirmation showed that the molar conversion rate of DCA was not more than 64% and the mass conversion rate was not more than 85%, when alkane was used as the single carbon source. Further analyses showed that the effective strategy to improve the conversion rate of alkane to DCA was the utilization of the other carbon sources, such as sucrose. ;

关 键 词：发酵 代谢动力学模型 长链二元酸 热带假丝酵母 

分 类 号：TQ921[轻工技术与工程—发酵工程]