在线凝胶渗透色谱-串联气质联用法测定植物油和猪肉中55种农药残留  

Determination of 55 pesticide residues in vegetable oil and animal tissues using on-line gel permeation chromatography coupled with gas chromatography-mass spectrometry

作　　者：吴卫东[1] 吴凤琪[1] 万志刚[1] 靳保辉[1] 陈波[1] 沈金灿[1] 吕杰 叶英 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳市食品安全检测技术研发重点实验室,深圳518045 [2]岛津企业管理(中国)有限公司,广州510010

出　　处：《食品安全质量检测学报》2014年第11期3400-3409,共10页Journal of Food Safety and Quality

基　　金：国家质检总局科技计划项目(2012IK188;2014IK118);深圳市基础研究计划(JC201105190972A)~~

摘　　要：目的建立植物油和动物组织中55种农药残留的快速检测方法。方法 样品用经改进的Qu ECh ERS方法提取净化,提取液采用在线凝胶渗透色谱-串联气质联用法(gel permeation chromatography coupled with gas chromatography-mass spectrometry,GPC-GC-MS)检测。结果在0.01~0.20 mg/L范围内各农药组分具有良好的线性关系,相关系数均在0.992以上,方法的加标回收率为60%~130%,55种农药测定低限范围为0.005~0.030mg/kg。结论 该方法操作简便、快速、灵敏、准确,适合于植物油和动物组织中55种农药残留的测定。Objective To establish a method for the simultaneous determination of 55 pesticide residues in vegetable oil and animal tissues. Methods The samples were extracted and purified by a modified QuEChERS method, and then the supernatant was analyzed by on-line gel permeation chromatography coupled with gas chromatography-mass spectrometry (GPC-GC-MS) system.Results The calibration curves of 55 pesticide residues were linear in the range of 0.01~0.20 mg/L with good correlation coefficients more than 0.992, The recoveries of the method ranged from 60% to 130%, The limits of quantification were 0.005~0.03 mg/kg.ConclusionThe method is simple, rapid and reliable and can meet the requirement the simultaneous determination and analysis of 55 pesticide residues in vegetable oil and animal tissues.

关 键 词：在线凝胶渗透色谱-串联气质联用法 植物油 猪肉 农药残留 

分 类 号：O657.63[理学—分析化学] TS225.1[理学—化学] TS251.51[轻工技术与工程—粮食、油脂及植物蛋白工程]