QuEChERS-酶联免疫快速检测法测定茶叶中黄曲霉毒素B_1  

Simultaneous determination for aflatoxin B_1 in tea leaf by QuEChERS-enzyme-linked immunosorbent assay

作　　者：刘辉 张燕[1] 

机构地区：[1]广东产品质量监督检验研究院,佛山528300 [2]华南农业大学食品学院广东省食品质量安全重点实验室农业部农产品贮藏保鲜质量安全风险评估实验室,广州510642

出　　处：《食品安全质量检测学报》2015年第4期1307-1313,共7页Journal of Food Safety and Quality

基　　金：广东省科技计划项目(2013B090600059)~~

摘　　要：目的建立QuEChERS-酶联免疫快速检测茶叶中黄曲霉毒素B1的方法,并对样品前处理条件进行优化。方法茶叶样品用70%乙腈水提取溶液进行提取目标物,离心后取上清液并用PSA+MgSO4进行净化处理后,采用酶联免疫快速检测方法进行分析测定。结果本方法的检出限为0.078μg/kg,线性范围为0.125~0.854μg/kg,IC50值为0.327 ng/m L。在三个不同添加水平下,样品的平均回收率为87.66%~97.17%,相对标准偏差为4.89%~7.16%。检测结果与高效液相色谱法(high performance liquid chromatography,HPLC)方法的相关系r2=0.9854,线性相关性良好。结论该方法更加简便、快速、高效,能够用于茶叶中黄曲霉毒素B1的检测。Objective To establish a QuEChERS-enzyme-linked immunosorbent assay(ELISA) method for determining the aflatoxin B1(AFB1) in tea leaf rapidly, and optimizing the sample pre-treatment conditions. Methods The samples were extracted with 70% acetonitrile in water, and the supernatant liquid was directly purified with PSA+Mg SO4 for detection of ELISA. Results The limit of detection(LOD) of method was 0.078 μg/kg, with linear range between 0.125 and 0.854 μg/kg, and IC50 value was 0.327 ng/m L. Average recovery rate of samples was between 87.66% and 97.17% at 3 adding levels, and the relative standard deviation(RSD) was 4.89%~7.16%. The results showed a good coefficient with high performance liquid chromatography(HPLC)(r2=0.9854). Conclusion This method proved to be suitable for the screening of tea leaf samples for the presence of AFB1, which was easier, faster and more efficient.

关 键 词：QUECHERS 酶联免疫法 黄曲霉毒素B1 茶叶 

分 类 号：TS272.7[农业科学—茶叶生产加工]